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Thiamine hcl

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Thiamine HCl is a chemical compound that serves as a source of vitamin B1. It is a water-soluble, crystalline solid that is commonly used in various laboratory applications.

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Lab products found in correlation

Soluble starch, by Merck Group (6 mentions) Vitamin b12, by Merck Group (6 mentions) Sodium chloride (nacl), by Carl Roth (6 mentions) 4 aminobenzoic acid, by Merck Group (6 mentions) K2hpo4, by Carl Roth (6 mentions) Kh2po4, by Carl Roth (6 mentions) L glutamine, by Carl Roth (6 mentions) Thiamine pyrophosphate, by Merck Group (6 mentions) D glucose, by Carl Roth (6 mentions) Proteose peptone no 3, by BD (5 mentions) Fe no3 3, by Merck Group (5 mentions) β nicotinamide adenine dinucleotide, by Carl Roth (5 mentions) Bacto agar, by BD (3 mentions) L cysteine hcl h20, by Carl Roth (3 mentions) Dehydrated agar, by BD (3 mentions) Isovitalex, by Carl Roth (2 mentions) L cysteine hcl h2o, by Carl Roth (2 mentions) Streptomycin, by Merck Group (1 mentions) Erythromycin, by Thermo Fisher Scientific (1 mentions) Chloramphenicol, by Merck Group (1 mentions)

6 protocols using thiamine hcl

1

Cultivation of Neisseria gonorrhoeae and E. coli

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Neisseria gonorrhoeae was grown overnight at 37°C and 5% CO2 on agar plates containing gonococcal base (GC) agar [10 g/l Bacto agar (BD Biosciences, Bedford, MA, United States), 5 g/l NaCl (Roth, Darmstadt, Germany), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD), 0.5 g/l soluble starch (Sigma-Aldrich, St. Louis, MO, United States)] supplemented with IsoVitaleX (IVX): 1 g/l D-Glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL × H20 (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth) and 0.1 mg/l vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
E. coli was grown in LB (Lysogeny Broth, Roth) medium or on LB agar plates (15 g/l Bacto agar (BD Biosciences, Bedford, MA, United States) at 37°C.
For N. gonorrhoeae antibiotics were used at the following concentrations: 2.5–5 μg/ml erythromycin (Thermo-Fisher), 100 μg/ml streptomycin (Sigma-Aldrich), 10 μg/ml chloramphenicol (Sigma-Aldrich). For E. coli antibiotics were used at the following concentrations: 50 μg/mL kanamycin (Roth).
Kraus-Römer S., Wielert I., Rathmann I., Grossbach J, & Maier B. (2022). External Stresses Affect Gonococcal Type 4 Pilus Dynamics. Frontiers in Microbiology, 13, 839711.
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2

Gonococcal Base Agar Composition

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Gonococcal base agar was made from 10 g/l dehydrated agar (BD Biosciences, Bedford, MA), 5 g/l NaCl (Roth, Darmstadt, Germany), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD Biosciences), 0.5 g/l soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/l D-glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL x H2O (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/l vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
Cronenberg T., Hennes M., Wielert I, & Maier B. (2021). Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment. PLoS Pathogens, 17(2), e1009251.
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3

Culturing N. gonorrhoeae for Experiments

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N. gonorrrhoeae (Table S1) was grown overnight at 37°C and 5% CO2 on agar plates containing gonococcal base agar (10 g/l Bacto agar (BD Biosciences, Bedford, MA, USA), 5 g/l NaCl (Roth, Darmstadt, Germany), 4 g/l K2HPO4 (Roth), 1 g/l KH2PO4 (Roth), 15 g/l Proteose Peptone No. 3 (BD), 0.5 g/l soluble starch (Sigma-Aldrich, St. Louis, MO, USA)) and the following supplements: 1 g/l D-Glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL×H20 (Roth), 1 mg/l thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth) and 0.1 mg/l vitamin B12 (Sigma-Aldrich). Before each experiment gonococcal colonies were resuspended in GC-medium.
Gangel H., Hepp C., Müller S., Oldewurtel E.R., Aas F.E., Koomey M, & Maier B. (2014). Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation. PLoS Pathogens, 10(4), e1004043.
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4

Gonococcal Base Agar Preparation

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Media composition, assay preparation, and setup follow [27 (link)]. Gonococcal base agar was made from 10 g/L dehydrated agar (BD Biosciences, Bedford, Massachusetts, United States of America), 5 g/L NaCl (Roth, Darmstadt, Germany), 4 g/L K2HPO4 (Roth), 1 g/L KH2PO4 (Roth), 15 g/L Proteose Peptone No. 3 (BD Biosciences), 0.5 g/L soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/L D-glucose (Roth), 0.1 g/L L-glutamine (Roth), 0.289 g/L L-cysteine-HCL × H2O (Roth), 1 mg/L thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/LFe(NO3) 3 (Sigma-Aldrich), 0.03 mg/L thiamine HCl (Roth), 0.13 mg/L 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/L β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/L vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch. Employed bacterial strains (Table i in S1 Text) are derivatives of strain N. gonorrhoeae MS11.
Hennes M., Bender N., Cronenberg T., Welker A, & Maier B. (2023). Collective polarization dynamics in bacterial colonies signify the occurrence of distinct subpopulations. PLOS Biology, 21(1), e3001960.
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5

Preparation of GC-base Agar Medium

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GC base agar was made from 10 g/L dehydrated agar (BD Biosciences, Bedford, MA), 5 g/L NaCl (Roth, Darmstadt, Germany), 4 g/L K2HPO4 (Roth), 1 g/L KH2PO4 (Roth), 15 g/L Proteose Peptone No. 3 (BD Biosciences), 0.5 g/L soluble starch (Sigma-Aldrich, St. Louis, MO), and supplemented with 1% IsoVitaleX (IVX): 1 g/L D-glucose (Roth), 0.1 g/L L-glutamine (Roth), 0.289 g/L l-cysteine-HCL × H2O (Roth), 1 mg/L thiamine pyrophosphate (Sigma-Aldrich), 0.2 mg/L Fe(NO3)3 (Sigma-Aldrich), 0.03 mg/L thiamine HCl (Roth), 0.13 mg/L 4-aminobenzoic acid (Sigma-Aldrich), 2.5 mg/L β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/L vitamin B12 (Sigma-Aldrich). GC medium is identical to the base agar composition but lacks agar and starch.
Welker A., Hennes M., Bender N., Cronenberg T., Schneider G, & Maier B. (2021). Spatiotemporal dynamics of growth and death within spherical bacterial colonies. Biophysical Journal, 120(16), 3418-3428.
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6

Cultivation of Neisseria gonorrhoeae

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N. gonorrrhoeae was grown overnight at 37°C and 5% CO2 on agar plates containing gonococcal base agar (10 g/l Bacto agar [BD Biosciences, Bedford, MA, USA], 5 g/l NaCl [Roth, Darmstadt, Germany], 4 g/l K2HPO4 [Roth], 1 g/l KH2PO4 [Roth], 15 g/l Bacto Proteose Peptone No. 3 [BD], 0.5 g/l soluble starch [Sigma–Aldrich, St. Louis, MO, USA]), and the following supplements: 1 g/l D-Glucose (Roth), 0.1 g/l L-glutamine (Roth), 0.289 g/l L-cysteine-HCL × H20 (Roth), 1 mg/l thiamine pyrophosphate (Sigma–Aldrich), 0.2 mg/l Fe(NO3)3 (Sigma–Aldrich), 0.03 mg/l thiamine HCl (Roth), 0.13 mg/l 4-aminobenzoic acid (Sigma–Aldrich), 2.5 mg/l β-nicotinamide adenine dinucleotide (Roth), and 0.1 mg/l vitamin B12 (Sigma–Aldrich). Before each experiment, gonococcal colonies were resuspended in GC-medium.
Oldewurtel E.R., Kouzel N., Dewenter L., Henseler K, & Maier B. (2015). Differential interaction forces govern bacterial sorting in early biofilms. eLife, 4, e10811.
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