Anti snai2

Manufactured by Abcam

Sourced in United Kingdom

Anti-SNAI2 is a laboratory reagent used for detecting and measuring the SNAI2 (Snail Family Transcriptional Repressor 2) protein in biological samples. It is designed for research use only and its core function is to facilitate the study of SNAI2 expression and regulation.

Automatically generated - may contain errors

Lab products found in correlation

Anti fxr, by Abcam (2 mentions) Anti β actin, by Bioworld Technology (2 mentions) Anti hdac6, by Cell Signaling Technology (2 mentions) Anti cdx2, by Cell Signaling Technology (2 mentions) Anti hnf4α, by Abcam (2 mentions) Anti lox antibody, by Abcam (1 mentions) Imagescope software, by Leica (1 mentions) Ripa buffer, by Beyotime (1 mentions)

3 protocols using anti snai2

Immunohistochemical Analysis of Thyroid Cancer Markers

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Sections were deparaffinized and rehydrated, and antigen retrieval was performed with citrate buffer in a water bath at 120°C. The sections were incubated with the anti-LOX antibody (1:100; Abcam), anti-SNAI2 or anti-TIMP4 (1:100, Abcam) overnight at 4°C, followed by incubation with a biotinylated secondary antibody for 1 hour at room temperature. The slides were developed with diaminobenzidine [DAB; EnVision+ Kit system HRP (DAB), Dako] and counterstained with hematoxylin. The slides were scanned at a 20× magnification using a ScanScope XT digital slide scanner (Aperio Technologies, Leica) to create whole-slide image data files at a resolution of 0.5 μm/pixel, and they were viewed using ImageScope software (Aperio Technologies).
Tissue microarrays were purchased from US Biomax (#TH641). These arrays included duplicates of six follicular adenomas, six follicular thyroid carcinomas, six papillary thyroid carcinomas, six anaplastic thyroid carcinomas, and 16 normal tissues from lung, thyroid, and testis.

Boufraqech M., Zhang L., Nilubol N., Sadowski S.M., Kotian S., Quezado M, & Kebebew E. (2016). Lysyl Oxidase (LOX) Transcriptionally Regulates SNAI2 Expression and TIMP4 Secretion in Human Cancers. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(17), 4491-4504.

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Western Blot Analysis of Protein Expression

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Total protein was extracted by mixing cells with radioimmunoprecipitation assay (RIPA) buffer (#P0013B, Beyotime, China), which was supplemented with protease and phosphatase inhibitors. Next, the proteins were separated by SDS-PAGE and transferred to NC membranes (#66485, Pall Inc., USA). The resulting membranes were incubated with 5% milk/TBST for 2 h at room temperature. Next, TBST was used to wash the membranes for 3 × 5 min, after which they were incubated with primary antibodies at 4 °C overnight. Finally, the membranes were incubated with HRP-conjugated secondary antibodies (#SAG10002, AntiProtech Inc., USA) after washing in TBST for 3 × 7 min. The following antibodies were used: anti-FXR (#187735, 1:200, Abcam, UK), anti-SNAI2 (#106077, 1:1000, Abcam, UK), anti-HDAC6 (#7558, 1:1000, Cell Signaling Technology, USA), anti-HNF4α (#92378, 1:2000, Abcam, UK), anti-CDX2 (#12306, 1:1000, Cell Signaling Technology, USA), and anti-β-actin (#AP0060, 1:5000, Bioworld, China). The primary antibody dilution buffer, PREstain Protein Ladder and Western SuperSensitive Substrate were purchased from BioCytoSci (#IC-8008, #IC-8001, China).

Wang N., Wu S., Zhao J., Chen M., Zeng J., Lu G., Wang J., Zhang J., Liu J, & Shi Y. (2021). Bile acids increase intestinal marker expression via the FXR/SNAI2/miR-1 axis in the stomach. Cellular Oncology (Dordrecht), 44(5), 1119-1131.

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Western Blot Analysis of Cell Proteins

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Total protein was extracted by mixing cells with radioimmunoprecipitation assay (RIPA) buffer (Beyotime, China), which was supplemented with protease and phosphatase inhibitors. Western blot analysis was carried out following standard procedures. The following antibodies were used for this experiment: anti-FXR (1:200, abcam, #187735), anti-SNAI2 (1:1000, abcam, #106077), anti-HDAC6 (1:1000, Cell Signaling Technology, #7558), anti-HNF4α (1:2000, Abcam, #92378), anti-CDX2 (1:1000, Cell Signaling Technology, #12306), and anti-β-actin (1:5000, Bioworld, #AP0060). The primary antibody dilution buffer, PREstain Protein Ladder and Western SuperSensitive Substrate were purchased from BioCytoSci (USA).

Wang S., Chen M., Zeng J., Lu G., Wang J., Zhang J., Wu S., & Shi Y. (2020). Bile acids increase intestinal markers via FXR/SNAI2/miR-1 axis in the stomach.

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