Anti phospho akt thr308 clone c31e5e

Manufactured by Cell Signaling Technology

The Anti-Phospho-Akt (Thr308) clone C31E5E is a rabbit monoclonal antibody that specifically recognizes Akt phosphorylated at threonine 308. This antibody can be used to detect the activated form of Akt in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti phospho erk1 2 thr202 tyr204 clone e10, by Cell Signaling Technology (1 mentions) Facsverse cytometer, by BD (1 mentions) Facscanto 2, by BD (1 mentions)

Spelling variants of this product

Anti-AKT (1447 citations) Phospho-Akt (892 citations) Anti-phospho-Akt (493 citations) Phospho-AKT (Thr308) (121 citations) Anti-phospho-AKT (Thr308) (53 citations)

2 protocols using anti phospho akt thr308 clone c31e5e

Phospho-Akt and Phospho-Erk1/2 Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 2% formaldehyde (Applichem Lifescience), permabilized with 100% methanol (VWR BDH Prolabo), and stained with anti-Phospho-Akt (Thr308) clone C31E5E and anti-Phospho-Erk1/2 (Thr202/Tyr204) clone E10 from Cell Signaling Technologies following manufacturers' recommendations. RPE-coupled goat anti-mouse and anti-rabbit secondary antibodies (Life Technologies) were used and samples were acquired in a FACSVerse cytometer (BD). FCS files were analyzed in FlowJo software (TriStar).

Moreno-Martínez D., Nomdedeu M., Lara-Castillo M.C., Etxabe A., Pratcorona M., Tesi N., Díaz-Beyá M., Rozman M., Montserrat E., Urbano-Ispizua Á., Esteve J, & Risueño R.M. (2014). XIAP inhibitors induce differentiation and impair clonogenic capacity of acute myeloid leukemia stem cells. Oncotarget, 5(12), 4337-4346.

+ Open protocol

+ Expand

Phospho-Akt Immunostaining and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed, permeabilized, and stained with anti-Phospho-Akt (Thr308) clone C31E5E from Cell Signaling Technologies following manufacturers' recommendations. RPE-coupled anti-rabbit secondary antibodies (Life Technologies) were used and samples were acquired in a FACSCanto II (BD).

Cornet-Masana J.M., Moreno-Martínez D., Lara-Castillo M.C., Nomdedeu M., Etxabe A., Tesi N., Pratcorona M., Esteve J, & Risueño R.M. (2016). Emetine induces chemosensitivity and reduces clonogenicity of acute myeloid leukemia cells. Oncotarget, 7(17), 23239-23250.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!