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2 protocols using anti p p65 ab

1

Neutrophil Activation and Inflammation

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Anti-CaSR-Ab was purchased from NOVUS (Colorado, USA). Anti-P-65-Ab and anti-P-p65-Ab were from Cell Signaling Technology (Boston, USA). Histopaque1083, Histopaque1119, and fMLP were from Sigma (Darmstadt, Germany). NPS-2143, cincalcet, and PDTC were purchased from MCE (New Jersey, USA). Rat IL-6 ELISA kit was from Elabscience (Wuhan, China), rat MPO ELISA kit was from USCN (Wuhan, China), and rat IL-10 was from CUSABIO (Wuhan, China). DHE-ROS kit was from BestBio (Shanghai, China), and NO assay kit was from Beyotime (Shanghai, China).
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2

T Cell Apoptosis Signaling Pathways

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Anti-CaSR-Ab was purchased from Alomone Labs (Jerusalem, Israel). Anti-Caspase-12-Ab was from Abcam (Cambridge, UK). Anti-P-p65-Ab was from Cell Signaling Technology (Boston, MA, USA). Plasmid was purchased from Life Technologies (Carlsbad, CA, USA). Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD and LEGENDplexTM Multi-analyte Flow Assay Kit were from BioLegend (San Diego, CA, USA). RosetteSep™ Human T cell enrichment cocktail was from Stem Cell Technologies (Vancouver, WA, USA). High Pure RNA Isolation Kit and Transcriptor First Strand cDNA Synthesis Kit was from Roche (Basel, Switzerland). VPA-1002 Human T Cell Nucleofector Kit 25 test was purchased from Lonza (Basel, Switzerland).
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