Biphasic nnn schneider s insect medium

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Biphasic NNN/Schneider's Insect Medium is a laboratory culture medium used for the cultivation and maintenance of various insect cell lines and parasites. It is a combination of the Novy-MacNeal-Nicolle (NNN) medium and Schneider's Insect Medium, providing the necessary nutrients and growth factors for the targeted organisms.

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Schneider’s insect medium (223 citations) Schneider’s medium (137 citations) Schneider medium (23 citations) Schneider insect medium (12 citations)

2 protocols using biphasic nnn schneider s insect medium

Leishmania Promastigote Cultivation and Identification

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The samples were seeded onto biphasic NNN/Schneider's Insect Medium (Sigma‐Aldrich Co, St Louis, MO) containing 10% fetal bovine serum and incubated at 26 ± 28°C23 for at least 30 days. The Leishmania promastigotes, isolated from tissues, were further identified at the species level by MLEE using five enzymatic systems according to protocols previously described.24

da Silva A.V., de Souza T.L., Figueiredo F.B., Mendes AA J.r., Ferreira L.C., Filgueira C.P., Cuervo P., Porrozzi R., Menezes R.C, & Morgado F.N. (2020). Detection of amastigotes and histopathological alterations in the thymus of Leishmania infantum‐infected dogs. Immunity, Inflammation and Disease, 8(2), 127-139.

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Isolation and Identification of Leishmania infantum

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The samples were seeded onto biphasic NNN/Schneider’s Insect Medium (Sigma-Aldrich Co., St. Louis, MO, USA) containing 10% fetal bovine serum and incubated at 26–28°C [29 (link)]. The Leishmania promastigotes isolated from skin, spleen, bone marrow and lymph nodes were identified as L. infantum by MLEE using five enzymatic systems [30 (link)]. These tests were performed at the Laboratório de Vigilância em Leishmanioses, INI, Fiocruz, which is a referral center for leishmaniasis of the Brazilian Ministry of Health.

Oliveira V.D., Boechat V.C., Mendes Junior A.A., Madeira M.D., Ferreira L.C., Figueiredo F.B., Campos M.P., de Carvalho Rodrigues F.D., Carvalhaes de Oliveira R.D., Amendoeira M.R, & Menezes R.C. (2017). Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations. PLoS ONE, 12(4), e0175588.

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