For quantitative evaluation of P-gp expression, the mean fluorescence channel (MFC) was calculated by FCS5 express software (De Novo software). P-gp content in arbitrary units was expressed as the ratio of the MFC of labeled resistant cells on the MFC of parental ones.
Fcs5 express software
FCS5 express software is a data analysis tool for flow cytometry data. It provides users with capabilities to import, visualize, and analyze flow cytometry data files.
Lab products found in correlation
4 protocols using fcs5 express software
Flow Cytometric Analysis of P-gp Expression
For quantitative evaluation of P-gp expression, the mean fluorescence channel (MFC) was calculated by FCS5 express software (De Novo software). P-gp content in arbitrary units was expressed as the ratio of the MFC of labeled resistant cells on the MFC of parental ones.
Evaluating Immune Phenotype and Docetaxel Response in Prostate Cancer
To assess the effects of Docetaxel treatment, PC3, PC3RR, DU-145 and DU-145RR cells were seeded 9 × 104/well and 6 × 104/well, respectively, in 12-well plates, then treated with different concentrations (from 10 nM to 100 nM) of Docetaxel (Sigma-Aldrich) for 24 and 48 h. Then, apoptosis/necrosis were assayed by using PI/Annexin Pacific Blue staining (Thermo Fisher Scientific, Rockford, IL, USA) and evaluated using flow cytometry.
CD44v9 Expression and Cell Cycle Analysis
Measurement of Cellular Reactive Oxygen Species
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