Trachea and lung tissues were fixed in 10% neutral buffered formalin (~7–10 days) then routinely processed, paraffin-embedded, sectioned (~4 µm) and stained with H&E and dPAS stains. Tissues were examined by a pathologist (DKM) masked to conditions. Digital images were collected with specialized equipment (BX51 microscope and DP73 digital camera, Olympus) and software (CellSens Pathology Edition, Olympus). Indices of inflammation were assigned as previously described [12 (link)]. Histological examination included 3 animals per treatment group that did not undergo flexiVent procedures, in addition to those that did.
Dp73 digital
Manufactured by Olympus
Sourced in Germany, Japan, United States
The DP73 digital is a high-performance microscope camera designed for scientific and industrial applications. It features a 17.28-megapixel CMOS sensor and can capture images with a resolution of up to 4800 x 3600 pixels. The camera is capable of live image display at up to 60 frames per second and supports various file formats, including TIFF, JPEG, and AVI. The DP73 digital is compatible with a wide range of microscope systems and can be controlled through a computer using the provided software.
Automatically generated - may contain errors
Lab products found in correlation
Cellsens pathology edition, by Olympus (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Autostainer link 48, by Agilent Technologies (1 mentions)
Fluorol yellow 088, by Santa Cruz Biotechnology (1 mentions)
Syto 24 green fluorescent nucleic acid stain, by Thermo Fisher Scientific (1 mentions)
Bx53 microscope, by Olympus (1 mentions)
Bx46 light microscope, by Olympus (1 mentions)
Cellsens standard 1, by Olympus (1 mentions)
6 protocols using dp73 digital
1
Histopathological Evaluation of Lung Tissues
2
Measuring Cleaved Caspase 3 in Kidney Tissue
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Immunohistochemical assay was used to the measure the expression of cleaved Caspase 3 in kidney slices. The kidneys were fixed, paraffin-embedded and cut into 3 μm slices. After being dewaxed and hydrated, the tissue slices were sequentially incubated with 3% hydrogen peroxide (H2O2) to block endogenous peroxidase activity, with albumin bovine fraction for blocking, and with anti-cleaved Caspase 3 antibody (1:500) overnight. Followed by secondary antibody and 3,3’3′-Diaminobenzidine (DAB) kit, the samples were examined under Olympus IX71 microscope (bright-field) equipped with an Olympus DP73 digital camera. Ten fields were randomly selected for each sample. The brown color was considered as positive staining according to the standard protocol.
3
Quantifying DNA Damage in Mouse Tumors
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Anti-H2AX pSer139 (γH2AX, 1:800, Novus, Centennial, CO, USA) antibody was used in mouse paraffin-embedded tumors to determine DNA damage in the treated samples. IHC analyses were performed in a DAKO Autostainer Link48 following the manufacturer’s instructions. Representative images were obtained using an Olympus DP73 digital camera, and the γH2AX determination was carried out with the Image J software (1.8.0.172) with the “Colour Deconvolution Plugin” by adjusting the threshold to 100 and the “Analyze Particles Plugin” for detecting all stained areas. The total area was obtained using the ROI Manager.
4
Fluorol Yellow 088 Staining Periderm
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Sections were stained for 1 h with 0.005% Fluorol Yellow 088 (Santa Cruz Biotechnology, Texas, USA) [39 (link)] dissolved in 90% glycerol and melted polyethylene glycol 4000 (SERVA Electrophoresis, Heidelberg, Germany). The sections were transferred to the stage of a fluorescence microscope (BX-60 equipped with a DP 73 digital camera; Olympus and viewed in transmitted white light or under incident fluorescent light (filter U-MWB; 450–480 nm excitation; ≥520 nm emission wavelength; Olympus, Hamburg, Germany). The minimum number of biological replicates was three. To confirm the occurrence of a periderm, a minimum of 50 sections through the whole block were examined.
5
Visualizing Parasite Nucleic Acids
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Parasites were washed with 1× phosphate-buffered saline (PBS; VWR) and mixed with 40% glycerol solution (vol/vol) in 1× PBS on poly-l -Lys-coated coverslips (Thermo Fisher Scientific). Slides were stained with SYTO 24 green fluorescent nucleic acid stain (Thermo Fisher Scientific), and cells were observed with an Olympus BX-53 microscope (Olympus, Tokyo, Japan) equipped with an Olympus DP73 digital camera. Images were taken with Olympus cellSens v.1.6 software, and merging was done in ImageJ v.1.51n (109 (link)).
6
Digitized Microscopic Images for Diagnostics
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Photomicrographs of diagnostically-relevant fields on the glass slides were captured using an Olympus BX46 light microscope (Center Valley, Pennsylvania, USA) with a mounted Olympus DP73 digital camera. Two jpeg images of each case were captured: One using the ×10 objective lens and one using the ×100 objective lens. The ×10 lens used was UPlanApo ×10/0.40 infinity/0.17, and the ×100 oil-immersion lens was UPlanSApo ×100/1.40 infinity/0.17/FN26.5. Images were captured using cellSens Standard 1.11 software (Center Valley, Pennsylvania, USA) (Olympus) with the following settings: Automatic exposure, 4800 × 3600 (pixel shift) resolution, and natural camera contrast. No postprocessing software was used on the digital images except to convert the jpegs to ScanScope virtual slide (.svs) files for online evaluation. No substantial loss in image quality was identified due to the conversion. For the ×100 photos each jpeg file was 1.4 ± 0.6 Mb in size, and each case took about 4.5 min to locate a diagnostically appropriate field and photograph.
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