Magna protein a beads

Manufactured by Merck Group

Magna protein A beads are a type of affinity chromatography resin used for the purification of antibodies and other proteins. The beads are composed of a cross-linked agarose matrix with covalently coupled Protein A, a bacterial cell wall protein that binds to the Fc region of immunoglobulins. This resin is commonly used in the biopharmaceutical industry for the purification of monoclonal antibodies and other therapeutic proteins.

Automatically generated - may contain errors

Lab products found in correlation

Total goat igg, by Merck Group (2 mentions)

Close spelling variants of this product

Protein A beads (62 citations) Magna ChIP Protein A/G Magnetic Beads (37 citations) Magna ChIP Protein A magnetic beads (14 citations) Magna ChIP Protein A+G Beads (9 citations) Magna Protein A+G Magnetic Beads (2 citations)

2 protocols using magna protein a beads

Chromatin Immunoprecipitation for Saa3 Promoter

Check if the same lab product or an alternative is used in the 5 most similar protocols

MODE-K cells were crosslinked in 1% formaldehyde in PBS for 3 min at room temperature, and quenched in 125 mM glycine at 4 °C for 10 min. Nuclei from fixed cells were pelleted and used for chromatin immunoprecipitation (Diagenode). Each reaction included chromatin from 5 × 10⁶ cells, 5 μg goat anti-RARβ (Santa Cruz) or total goat IgG (Millipore), and 20 μL Magna protein A beads (Millipore). Bound Saa3 promoter sequences were quantified using SYBR Green-based real-time PCR (target sequence and primers listed in SI Appendix, Table S4). Relative enrichment of the Saa3 promoter was calculated as the ratio of specific antibody pull-down to input DNA.

Gattu S., Bang Y.J., Pendse M., Dende C., Chara A.L., Harris T.A., Wang Y., Ruhn K.A., Kuang Z., Sockanathan S, & Hooper L.V. (2019). Epithelial retinoic acid receptor β regulates serum amyloid A expression and vitamin A-dependent intestinal immunity. Proceedings of the National Academy of Sciences of the United States of America, 116(22), 10911-10916.

+ Open protocol

+ Expand

ChIP-qPCR Analysis of RETN Promoter

Check if the same lab product or an alternative is used in the 5 most similar protocols

SZ95 cells were crosslinked in cell culture media with 1% formaldehyde for 8 minutes at room temperature, followed by 125 μM glycine at 4°C for 10 min to quench the reaction. Shearing of nuclear DNA was completed per manufacturer’s protocol (Diagenode). For each immunoprecipitation reaction 20 μl of Magna protein A beads (Millipore) and chromatin from 1 × 10⁷ cells was combined with 5 μg of goat anti-RAR (Santa Cruz) or total goat IgG (Millipore). Complexed RETN promoter sequences were quantified using SYBR Green-based real-time PCR. Primer sequence and primers are listed in Key Resources Table. The ratio of specific antibody pull-down to input DNA was used to calculate relative enrichment of the RETN promoter.

Harris T.A., Gattu S., Propheter D.C., Kuang Z., Bel S., Ruhn K.A., Chara A.L., Edwards M., Zhang C., Jo J.H., Raj P., Zouboulis C.C., Kong H.H., Segre J.A, & Hooper L.V. (2019). Resistin-like molecule α provides vitamin A-dependent antimicrobial protection in the skin. Cell host & microbe, 25(6), 777-788.e8.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!