R24541

Manufactured by ZenBio

Sourced in China

R24541 is a laboratory centrifuge designed for general-purpose applications. It features variable speed control, a compact footprint, and a rotor capable of accommodating multiple sample tubes or microplates.

Automatically generated - may contain errors

Lab products found in correlation

Ab1128, by Merck Group (1 mentions) Ab68481, by Abcam (1 mentions) Sa00002 1, by Proteintech (1 mentions) Sa00002 2, by Proteintech (1 mentions) Ab125066, by Abcam (1 mentions) Df6437, by Affinity Biosciences (1 mentions) Protein free rapid blocking buffer, by Ipsen (1 mentions) Ab269513, by Abcam (1 mentions) Ab175186, by Abcam (1 mentions) Df12141, by Affinity Biosciences (1 mentions)

2 protocols using r24541

Andrographolide Modulates Heme Oxygenase-1

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Commercial andrographolide (purity 99.67%) was purchased from APExBIO (N1855, USA), with high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) quality control. Other reagents included dimethyl sulfoxide (DMSO) (D8371, Solarbio, China), HO-1 inducer hemin (HY-19,424, MCE, China), hemoglobin (HB) (H828504, Macklin, China), iron (II) chloride (FeCl₂) (I837006, Macklin, China), and biliverdin (BV) (30,891, Merck, Germany). The primary antibodies included HO-1 (R24541, Zen-Bio, China), RSV (AB1128, Millipore, Germany), interferon regulatory factor 3 (IRF3) (ab68481, Abcam, UK), and GAPDH (60004-1-Ig, Proteintech Group, USA). The secondary antibodies included alkaline phosphatase-conjugated goat anti-mouse antibody (SA00002-1, Proteintech Group, USA), goat anti-rabbit antibody (SA00002-2, Proteintech Group, USA), and donkey anti-goat antibody (SA00002-3, Proteintech Group, USA).

Che S., Zhou N., Liu Y., Xie J, & Liu E. (2023). Andrographolide exerts anti-respiratory syncytial virus activity by up-regulating heme oxygenase-1 independent of interferon responses in human airway epithelial cells. Molecular Biology Reports, 50(5), 4261-4272.

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Western Blot Analysis of Hepatocyte Proteins

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Protein lysates, obtained from hepatocytes and liver specimens, were electrophoresed on 4–12% precast bis–tris gels (Genscript, Nanjing, China) and subsequently transferred onto polyvinylidene fluoride (PVDF) membranes. The membranes were then blocked using protein-free rapid blocking buffer (EpiZyme, Shanghai, China) and incubated with primary antibodies overnight at 4 °C. Antibodies used were as follows: Med1 (ab243893, Abcam), Nrf2 (D1Z9C) (12721S, CST), HO-1 (R24541, Zen BioScience), ACSL4 (DF12141, Affinity Biosciences), TfR1 (ab269513, Abcam), SLC7A11 (ab175186, Abcam), GPX4 (ab125066, Abcam), NQO1(DF6437, Affinity Biosciences) and β-Tubulin (66,240–1-Ig, Proteintech).

Lei Z.Y., Li Z.H., Lin D.N., Cao J., Chen J.F., Meng S.B., Wang J.L., Liu J., Zhang J, & Lin B.L. (2024). Med1 inhibits ferroptosis and alleviates liver injury in acute liver failure via Nrf2 activation. Cell & Bioscience, 14, 54.

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