Axi vision camera

A Zeiss Stemi 2000-C microscope was used to analyze the lipids, ROS accumulation and GUS signal, the images were saved as jpg files using an Axi-Vision Camera. Live-cell microscopy was performed on a Zeiss 700 confocal laser scanning microscope using a 488 nm emission filter to detect the GFP signal.

All of the explants derived from the lines with GUS constructs were stained in a standard X-Gluc (5-bromo-4-chloro-3-indolyl β-d-glucuronide cyclohexylammonium salt) (Sigma Aldrich) solution at 37 °C for 12 h as described by Jefferson et al. (1987 (link)). The expression of the GUS signal was inspected in the IZEs of the analysed lines using a Zeiss Stemi 2000-C microscope and images were saved as jpg files with an Axi-Vision Camera.
Green fluorescence protein (GFP) fluorescence was excited with a multiband argon laser at 100 mV (Melles Griot BV) and a wavelength of 488 nm. Image processing was performed using the FLUOVIEW computer program (Olympus, version 1.6).