Rpmi 1640 without nahco3 51800

Manufactured by Thermo Fisher Scientific

RPMI-1640 without NaHCO3 (51800) is a cell culture medium formulation designed for the in vitro cultivation of cells. It is a dry powder that requires supplementation and reconstitution prior to use.

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Lab products found in correlation

Sodium bicarbonate 25080, by Thermo Fisher Scientific (3 mentions) Dry milk 170 6404, by Bio-Rad (3 mentions) Pvdf membranes rpn303f, by GE Healthcare (3 mentions) Hrp conjugated anti rabbit na934v and anti mouse nxa931 antibodies, by GE Healthcare (2 mentions) Ecl system rpn2106, by GE Healthcare (2 mentions) β actin, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Verteporfin, by Merck Group (1 mentions) β actin actb, by Merck Group (1 mentions) Human recombinant epidermal growth factor, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

RPMI 1640 NaHCO3

3 protocols using rpmi 1640 without nahco3 51800

Protein Extraction and Western Blotting

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RPMI-1640 (SH30255.01), trypsin (SH40003.12), phosphate-buffered saline (PBS, SH40003.12) and Fetal Bovine Serum (FBS) (SV30160.03) were purchased from HyClone. Sodium Bicarbonate (25080) and RPMI-1640 without NaHCO₃ (51800) were purchased from Gibco. Bafilomycin A1 (BafA1, B1793), protease cocktail tablets EDTA-free, phosphatase inhibitors (P5726, P0044) and Verteporfin were purchased from Sigma. Protein assay dye reagent concentrate (500-0006), protein assay standard (5000-0007) and dry milk (170-6404) were purchased from Bio-Rad. The following antibodies were used: LC3B (Cell Signaling Technology, 2775), SQTM1/p62 (BD Biosciences, 610833) and β-actin (Sigma, A5441). HRP-conjugated anti-rabbit (NA934V) and anti-mouse (NXA931) antibodies, ECL system (RPN2106) and PVDF membranes (RPN303F) were purchased from GE Healthcare. The fluorogenic substrate Suc-LLVY-AMC (S-280) was purchased from Boston Biochem (USA).

Pellegrini P., Serviss J.T., Lundbäck T., Bancaro N., Mazurkiewicz M., Kolosenko I., Yu D., Haraldsson M., D’Arcy P., Linder S, & De Milito A. (2018). A drug screening assay on cancer cells chronically adapted to acidosis. Cancer Cell International, 18, 147.

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Autophagy Regulation in Cell Lines

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RPMI-1640 (SH30255.01), trypsin (SH40003.12), and phosphate-buffered saline (PBS, SH40003.12) were from HyClone. RPMI-1640 without NaHCO₃ (51800) and Sodium Bicarbonate (25080) were from Gibco. BafA1 (B1793), CQ diphosphate salt (C6628), HCQ (H0915), protease cocktail tablets EDTA-free, phosphatase inhibitors (P5726, P0044) and bovine serum albumin (A7906) were from Sigma. The Lys-01 was synthesized by OncoTargeting AB (Sweden) and dissolved in DMSO. Protein assay standard I (5000-0007) and dry milk (170-6404) were from Bio-Rad. The following antibodies were used: LC3B (Cell Signaling Technology, 2775), cathepsin B (CTSB) (Calbiochem, PC41) and β-actin (ACTB) (Sigma, A5441). HRP-conjugated anti-rabbit (NA934V) and anti-mouse (NXA931) antibodies, ECL system (RPN2106) and PVDF membranes (RPN303F) were from GE Healthcare.

Pellegrini P., Strambi A., Zipoli C., Hägg-Olofsson M., Buoncervello M., Linder S, & De Milito A. (2014). Acidic extracellular pH neutralizes the autophagy-inhibiting activity of chloroquine: Implications for cancer therapies. Autophagy, 10(4), 562-571.

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Autophagy Modulation in Cell Cultures

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RPMI-1640 (SH30255.01), trypsin (SH40003.12), phosphate-buffered saline (PBS, SH40003.12) and Fetal Bovine Serum (FBS) (SV30160.03) were from HyClone. Sodium Bicarbonate (25080) and RPMI-1640 without NaHCO₃ (51800) were from Gibco. Bafilomycin A1 (BafA1, B1793), Chloroquine di-phosphate salt (CQ, C6628), salinomycin (SAL, S4526), protease cocktail tablets EDTA-free, phosphatase inhibitors (P5726, P0044), insulin (I9278) and bovine serum albumin (BSA, A7906) were from Sigma. Recombinant human epidermal growth factor (EGF) was purchased from Invitrogen (PHG6045). The Lys-01 was synthetized by OncoTargeting AB (Sweden) and dissolved in DMSO. Protein assay dye reagent concentrate (500-0006), protein assay standard I (5000-0007) and dry milk (170-6404) were from Bio-Rad. The following antibodies were used: LC3B (Cell Signaling Technology, 2775) and β-actin (Sigma, A5441). HRP-conjugated anti-rabbit (NA934V) and anti-mouse (NXA931) antibodies, ECL system (RPN2106) and PVDF membranes (RPN303F) were from GE Healthcare.

Pellegrini P., Dyczynski M., Sbrana F.V., Karlgren M., Buoncervello M., Hägg-Olofsson M., Ma R., Hartman J., Bajalica-Lagercrantz S., Grander D., Kharaziha P, & De Milito A. (2016). Tumor acidosis enhances cytotoxic effects and autophagy inhibition by salinomycin on cancer cell lines and cancer stem cells. Oncotarget, 7(24), 35703-35723.

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