Anti rab7a

Protein expression was detected by western blotting, as previously described64 (link). Briefly, for cell lysis, RIPA buffer containing protease and phosphatase inhibitors was used. Proteins were separated by SDS–polyacrylamide gel electrophoresis electrophoresis, transferred to a PVDF membrane and detected with the cognate antibody. The following antibodies were used: anti-Pan Actin (1:10,000; Neomarkers, Fremont, CA); anti-total EGFR (1:1,000; sc-03, Santa Cruz, Dallas, TX); anti-pEGFR (1:1,000; ab5644, Abcam, Cambridge, MA); anti-HA tag (12CA5) (11 583 816 001, Roche, Germany). The anti-Rab7A (1:6,000; #R8779), anti-FLAG tag (1:10,000; #F1804) and anti-Tubulin (1:5,000; #T9026) antibodies were from Sigma-Aldrich (St Louis, MO). The anti-FLCN antibody (1:3,000; #3697), anti-pERK (1:2,000; #4370 and 1:2,000; #9101), anti-pS6 (1:2,000; #5364), anti-HA (1:2,000; #3724), anti-pMET (1:1,000; #3129), total MET (1:1,000; #3148) and IgG control (#3900) antibodies were from Cell Signaling Technology (Danvers, MA). Western blots were developed using the Bio-Rad ChemiDoc system and densitometry was analysed with BioRad Image Lab Software (Bio-Rad Laboratories, Hercules, CA). Uncropped scans of the most important blots are contained in Supplementary Fig. 6.