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Cytoseal xyl xylene based mounting media

Manufactured by Thermo Fisher Scientific
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Cytoseal XYL is a xylene-based mounting media used for the preparation of microscope slides. It is designed to provide a transparent, durable, and long-lasting mounting solution for specimens, enabling effective preservation and presentation of samples for examination and analysis.

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Lab products found in correlation

Eosin y, by Merck Group (4 mentions) Eclipse 90i microscope, by Nikon (1 mentions) Nis element, by Nikon (1 mentions)

Close spelling variants of this product

Xylene (170 citations) Cytoseal XYL (130 citations) Cytoseal (112 citations) Mounting media (34 citations) Cytoseal XYL mounting media (14 citations) Cytoseal mounting media (4 citations)

4 protocols using cytoseal xyl xylene based mounting media

1

Histopathological Tissue Staining Protocol

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Histology of sections was observed on stained 5-μm sections that were fixed, paraffin embedded, deparaffinized, and rehydrated, as mentioned previously. Then, they were stained with Hematoxylin Stain Solution, Gill 3 (Ricca Chemical Company, Pocomoke City, MD) and Eosin Y (Sigma-Aldrich). Sections were dehydrated in an increasing series of ethanol baths (70%, 95%, and 100%), cleared in xylene, and mounted with Cytoseal XYL xylene-based mounting media (Thermo Fisher Scientific, Waltham, MA). The H&E stains were used for histopathological assessment.
Kim C.K., Saxena M., Maharjan K., Song J.J., Shroyer K.R., Bialkowska A.B., Shivdasani R.A, & Yang V.W. (2019). Krüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells. Cellular and Molecular Gastroenterology and Hepatology, 9(4), 587-609.
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2

Hematoxylin and Eosin Staining Protocol

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Histology of sections was observed upon staining 5µm sections that were fixed, paraffin-embedded, deparaffinized and rehydrated as mentioned before. Then they were stained with Hematoxylin Stain Solution, Gill 3 (Ricca Chemical Company) and Eosin Y (Sigma-Aldrich). Sections were dehydrated in an increasing series of ethanol bath (70%, 95%, 100%), cleared in xylene and mounted with Cytoseal XYL xylene-based mounting media (Thermo Scientific, Cat. #8312-4).
de Sabando A.R., Wang C., He Y., García-Barros M., Kim J., Shroyer K.R., Bannister T.D., Yang V.W, & Bialkowska A.B. (2015). ML264 - a novel small-molecule compound that potently inhibits growth of colorectal cancer. Molecular cancer therapeutics, 15(1), 72-83.
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3

Histological Staining Protocol

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Histology of sections was observed on stained 5-μm sections that were fixed, paraffin embedded, deparaffinized, and rehydrated as mentioned previously. Then, they were stained with Hematoxylin Stain Solution, Gill 3 (Ricca Chemical Company: 3537–32) and Eosin Y (Sigma-Aldrich: HT110216). Sections were dehydrated in an increasing series of ethanol baths (70%, 95%, and 100%), cleared in xylene, and mounted with Cytoseal XYL xylene-based mounting media (Thermo Scientific: 8312–4). The H&E stains were used for histopathological assessment.
Yang V.W., Liu Y., Kim J., Shroyer K.R, & Bialkowska A.B. (2018). Increased genetic instability and accelerated progression of colitis-associated colorectal cancer through intestinal epithelium-specific deletion of Klf4. Molecular cancer research : MCR, 17(1), 165-176.
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4

Histological Staining Protocol for FFPE Tissues

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For H&E staining, five-micrometer FFPE sections of tissues were baked in a 65 °C oven overnight and subsequently deparaffinized in xylene, rehydrated by incubation in a decreasing ethanol bath series (100%, 95%, and 70%), and stained with Hematoxylin Stain Solution, Gill 3 (Ricca Chemical Company (Arlington, TX, USA): 3537-32) and Eosin Y (Sigma–Aldrich (Saint Louis, MO, USA): HT110216). Sections were dehydrated in an increasing series of ethanol baths (70%, 95%, and 100%), cleared in xylene, and mounted with Cytoseal XYL xylene-based mounting media (Thermo Scientific (Waltham, MA, USA): 8312-4). The H&E stains were used for histopathological assessment. Images were taken using a Nikon Eclipse 90i microscope (Nikon, Melville, NY, USA) and processed with NIS-Elements (Nikon, Melville, NY, USA).
M. Swamynathan M., Mathew G., Aziz A., Gordon C., Hillowe A., Wang H., Jhaveri A., Kendall J., Cox H., Giarrizzo M., Azabdaftari G., Rizzo R.C., Diermeier S.D., Ojima I., Bialkowska A.B., Kaczocha M, & Trotman L.C. (2023). FABP5 Inhibition against PTEN-Mutant Therapy Resistant Prostate Cancer. Cancers, 16(1), 60.
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