SureSelect Human All Exon V4, Human All Exon V4+UTR (Agilent Technologies, Santa Clara, CA, USA), or NimbleGen SeqCap EZ Exome V2 (Roche, Wilmington, MA, USA) kits were used for the WES library preparation. Sequencing was performed on the Illumina HiSeq platform with 100 PE or 150 PE format (Illumina, San Diego, CA, USA). Variant calling was performed using the Genome Analysis Toolkit (GATK) following the best practice pipeline 24 (link) and variant annotation was performed using ANNOVAR 25 (link). To control for the batch effect, joint variant calling was performed on all samples and only variants that are in the smaller set of the enrichment regions (SeqCap EZ Exome V2) among the multiple exome capture kits were considered (see Supplemental Methods for detailed steps and commands). The sequencing data is available at dbGaP under study accession phs001423.v2.p2.
Human all exon v4 utr
Manufactured by Agilent Technologies
Sourced in United States
The Human All Exon V4+UTR is a comprehensive gene capture panel designed to target the protein-coding regions (exons) and untranslated regions (UTRs) of the human genome. It provides broad coverage of the human transcriptome, enabling researchers to study the full breadth of genetic variation across coding and regulatory regions.
Automatically generated - may contain errors
2 protocols using human all exon v4 utr
1
Whole Exome Sequencing: Variant Calling and Annotation
2
Whole Exome Sequencing: Variant Calling and Annotation
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SureSelect Human All Exon V4, Human All Exon V4+UTR (Agilent Technologies, Santa Clara, CA, USA), or NimbleGen SeqCap EZ Exome V2 (Roche, Wilmington, MA, USA) kits were used for the WES library preparation. Sequencing was performed on the Illumina HiSeq platform with 100 PE or 150 PE format (Illumina, San Diego, CA, USA). Variant calling was performed using the Genome Analysis Toolkit (GATK) following the best practice pipeline 24 (link) and variant annotation was performed using ANNOVAR 25 (link). To control for the batch effect, joint variant calling was performed on all samples and only variants that are in the smaller set of the enrichment regions (SeqCap EZ Exome V2) among the multiple exome capture kits were considered (see Supplemental Methods for detailed steps and commands). The sequencing data is available at dbGaP under study accession phs001423.v2.p2.
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