Two cell lines were used in the present study. One was the RAW 264.7 mouse macrophage cell line purchased from Culture Collection and Research Center, Food Industry and Development Institute, Hsinchu, Taiwan. The cells were grown in Dulbecco’s modified Eagle medium (Sigma) supplemented with 10% fetal bovine serum (Gibco Laboratories, Grand Island, NY) in humidified atmosphere containing 5% CO2 at 37 °C. The second cell line was human neuroblastoma IMR-32 cells, obtained from the American Type Culture Collection (ATCC), which were cultured in minimum essential medium (MEM) (Genedire X, USA) supplemented with 10% fetal bovine serum and 1% sodium pyruvate at 37 °C in a controlled atmosphere of 5% CO2. After 2- to 3-day growth that filled up to 70–80% of a 10-cm culture dish, the IMR-32 cultures were subcultured or collected, and nuclear proteins were extracted for performing the electrophoretic mobility shift assay (EMSA).
Minimum essential medium (mem)
Manufactured by GeneDireX
Sourced in United States
The MEM (Microplate Electrophoresis Module) is a laboratory instrument designed for performing electrophoresis analysis on DNA, RNA, or protein samples in a microplate format. It provides a reliable and efficient method for separating and analyzing biomolecules.
Automatically generated - may contain errors
2 protocols using minimum essential medium (mem)
1
Culturing Mouse Macrophages and Human Neuroblastoma Cells
2
Culture and Extraction of Cell Lines
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Two cell lines were used in our studies. One was the RAW264.7 mouse macrophage cell lines, purchased from Culture Collection and Research Center, Food Industry and Development Institute, Hsinchu, Taiwan. Cells were grown in Dulbecco's Modified Eagle Medium (Sigma) supplemented with 10% fetal bovine serum (Gibco Laboratories, Grand Island, NY) in a humidified atmosphere containing 5% CO2 at 37 °C. The second cell line was human neuroblastoma IMR-32 cells, obtained from the American Type Culture Collection (ATCC), were cultured in minimum essential medium (MEM) (Genedire X, USA) supplemented with 10% fetal bovine serum and 1% sodium pyruvate at 37•C in a controlled of 5% CO2 atmosphere. After 2-to 3-day growth, up to 70-80% full of 10 cm culture dish, the IMR-32 cultures were subcultured or collected and extracted out the nuclear proteins for EMSA use.
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