Peroxidase and fluorophore conjugated secondary antibodies

A rabbit anti-CADM1 polyclonal antibody directed against the C-terminal 15-amino acid peptide was generated in our laboratory as described previously [24 (link)]. Other primary antibodies used targeted cytokeratin 7 (CK7) (mouse monoclonal OV-TL 12/30; Dako, Glostrup, Denmark), single-stranded DNA (ssDNA) (rabbit IgG; Immuno-Biological Laboratories Co., Ltd., Gunma, Japan), thyroid transcription factor one (TTF1) (mouse monoclonal; Leica Biosystems, Nussloch, Germany), surfactant protein A (SP-A) (mouse monoclonal; Leica Biosystems), a cleaved form of caspase-3 (rabbit polyclonal; Cell Signaling Technology, Danvers, MA, USA), and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) (Merck Millipore, Billerica, MA, USA). Peroxidase- and fluorophore-conjugated secondary antibodies were obtained from Amersham (Buckinghamshire, England) and Jackson ImmunoResearch (West Grove, PA, USA), respectively.

A rabbit anti-CADM1 polyclonal antibody directed against the C-terminal 15-amino acid peptide was generated in our laboratory and described previously [22] (link). Other primary antibodies targeted glucagon (mouse monoclonal; Sigma-Aldrich, St. Louis, MO, USA), insulin (guinea pig polyclonal; Dako, Glostrup, Denmark), and β-actin (mouse monoclonal AC-15; Sigma-Aldrich). Peroxidase- and fluorophore-conjugated secondary antibodies were obtained from Amersham (Buckinghamshire, England) and Jackson ImmunoResearch (West Grove, PA, USA), respectively. Phorbol 12-myristate 13-acetate (PMA) and trypsin were purchased from Wako Pure Chemical Industries (Osaka, Japan).

A rabbit anti-CADM1 polyclonal antibody directed against the C-terminal 15-amino acid peptide was generated in our laboratory as described previously [16 (link)]. Other primary antibodies targeting fluorescein isothiocyanate (FITC) (Ab19224; Abcam, Cambridge, UK) and β-actin (mouse monoclonal AC-15; Sigma-Aldrich) were used. Peroxidase- and fluorophore-conjugated secondary antibodies were obtained from Amersham (Buckinghamshire, UK) and Jackson ImmunoResearch (West Grove, PA, USA), respectively. 4’,6’-Diamidino-2-phenylindole (DAPI; Dojindo, Kumakoto, Japan) and silver stain reagents (Sigma-Aldrich) were used according to the manufacturer’s instructions. Phorbol 12-myristate 13-acetate (PMA) was used as described previously [11 (link)].