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Ab243634

Manufactured by Abcam
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Ab243634 is a laboratory product manufactured by Abcam. It is a key component used in various scientific applications. The core function of this product is to facilitate specific experimental procedures, although the detailed description of its intended use is not available.

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Lab products found in correlation

Ab2811, by Abcam (2 mentions) Sc 515387, by Santa Cruz Biotechnology (2 mentions) His tag, by Cell Signaling Technology (1 mentions) Gst tag, by Cell Signaling Technology (1 mentions) Ab125243, by Abcam (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Ab150081, by Abcam (1 mentions) Ab150160, by Abcam (1 mentions)

2 protocols using ab243634

1

Co-immunoprecipitation of Protein Complexes

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Co‐immunoprecipitation was undertaken as documented,46, 47, 48, 49, 52, 53 using antibodies against CNBP (sc‐515387, Santa Cruz Biotechnology, Inc.), KPNB1 (ab2811), SMARCC2 (ab243634), Flag‐tag (ab125243, Abcam Inc.), Myc‐tag (#2276), His‐tag (#9991) or GST‐tag (#2624, Cell Signaling Technology). After being released from the bead‐bound complex, proteins were determined via western blotting or mass spectrometric assays (Wuhan SpecAlly Life Technology Co., Ltd., China).46, 47, 52, 53
Hu A., Chen G., Bao B., Guo Y., Li D., Wang X., Wang J., Li Q., Zhou Y., Gao H., Song J., Du X., Zheng L, & Tong Q. (2023). Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity. Clinical and Translational Medicine, 13(4), e1235.
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2

Immunofluorescence Staining of Cellular Proteins

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Cells were cultured on coverslips, which were treated by 10% goat serum for an hour at 37°C. Then, cells were treated by antibodies against KPNB1 (ab2811, Abcam Inc., dilution: 1:100), CNBP (sc‐515387, Santa Cruz Biotechnology, Inc., dilution: 1:100) or SMARCC2 (ab243634, Abcam Inc., dilution: 1:100) for 2 h. Coverslips were stained by 4′,6‐diamidino‐2‐phenylindole (300 nmol L−1) after incubating with Alexa Fluor 488 (ab150081) or Alexa Fluor 594 (ab150160, Abcam Inc.) goat anti‐rabbit IgG.
Hu A., Chen G., Bao B., Guo Y., Li D., Wang X., Wang J., Li Q., Zhou Y., Gao H., Song J., Du X., Zheng L, & Tong Q. (2023). Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity. Clinical and Translational Medicine, 13(4), e1235.
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