Ab213656

This study was approved by the Ethics Committee of the Second Affiliated Hospital of Chongqing Medical University and acquired informed consent from all patients who participated in this study. All methods were performed in accordance with the Helsinki declaration guidelines and regulations. The tissue samples of 20 patients with LIHC were obtained from the Second Affiliated Hospital of Chongqing Medical University. Recombinant anti-Annexin A10 was purchased from Abcam. Tissues embeded in paraffin were sliced into 3 µm thick sections. Antigen was repaired by sodium citrate solution (Beyotime, China). Immunohistochemistry (IHC) was carried out with the detection system of polymer horseradish peroxidase (Zhongshan Goldenbridge Biotechnology, China). The incubation of sections is the following specific primary antibodies: Anxa10(Abcam, ab213656, 1:1000).

Antibodies against ANXA10 (ab213656), β-Actin (ab8227), LC3B (ab192890), and SQSTM1 (ab109012) were purchased from Abcam (USA). Rabbit anti-β-Tubulin (#2128) antibody was purchased from Cell Signaling Technology. Rabbit anti-TFRC (10084-2-AP) was purchased from Proteintech, and mouse anti-Flag (DYKDDDDK-Tag) was purchased from Abmart. LAMP1 antibody (sc-20011) was purchased from Santa Cruz (CA, USA). Alexa Fluor 488 (A-21202) and Alexa Fluor 594 (A-21207) conjugated secondary antibodies were purchased from Invitrogen (CA, USA). Ferrostatin-1 (HY-100579), Deferoxamine mesylate (HY-B0988), Rapamycin (HY-10219), and Chloroquine (HY-17589A) were purchased from MedChemExpress. Cycloheximide (S7418) was purchased from Selleck, and DMSO (D2650) and N-acetyl-L-cysteine (A9165) were purchased from Sigma-Aldrich. Hydrogen peroxide solution (88597) was purchased from Millipore.