Protein was evaluated by 12% Bis‐Tris Gel/MOPS (Invitrogen) in MOPS SDS running buffer (Thermo Fisher) for 2 hours. After transferring the protein electrophoretically for 2 hours, the gels were transferred onto the PVDF membranes (Millipore), and were incubated with 5% skimmed milk (2321000; Becton, Dickinson and Company, America) for 1.5 hours. After that, the primary antibodies (Table S1 ) were added and maintained at 4°C overnight followed by the addition of secondary antibodies (Zhongshan Jinqiao) for 1.5 hours. Immunoreactive bands were analyzed by BiolD software (Vilber Lourmat).
Biold software
Manufactured by Vilber
Sourced in France
BiolD is a software tool that enables the identification of protein-protein interactions. It provides functionalities for data analysis and visualization of proximity-dependent biotinylation experiments.
Automatically generated - may contain errors
2 protocols using biold software
1
Protein Electrophoresis and Western Blot
2
Protein Expression Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The protein concentration was determined strictly in accordance with the Pierce BCA Assay Kit (Thermo Scientific, USA) instructions. Samples were electrophoresed on 12% Bis–Tris Gel/MOPS (Invitrogen, NY, USA) for 2 h, and transferred to PVDF membranes (Immobilon, Millipore, USA) for 1.5 h. The membranes were incubated with 5% skimmed milk (2321000, BD, USA) for 1.5 h, incubated with the primary and secondary antibodies (Table S2 ). The antibodies for GAPDH9 (link), Bcl-29 (link), Bax9 (link), β-catenin9 (link), and p7TP317 (link) has been used in previous experiments. The interested bands were analyzed by BiolD software (Vilber Lourmat, France). The blots were cut prior to hybridisation with antibodies. Therefore, original images of full-length blots cannot be provided. We have provided all original blots, with membrane edges visible, in supplementary materials.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!