Cell culture medium and reagents

Type III recombinant human collagen (Mw. 112 kD, The National Center for Biotechnology Information GenBank Access Number: EF376007.1) with high hydrophilicity was prepared by our laboratory [39 (link)]. Methacryllc anhydride (MAA, 94%, containing 0.2% topanol stabilizer) was purchased from Aladdin (Shanghai, China). Chitosan (Mw. 100-300 KD) was purchased from J&K Scientific (Beijing, China). Lithium phenyl-2,4,6-trimethylbenzoyl phosphinate (LAP) was purchased from Macklin. The Cell Counting Kit-8 reagent kit (CCK-8) was purchased from Beyotime Biotechnology (Shanghai, China). The acridine orange/ethidium bromide (AO/EB) assay kit was purchased from Yeasen Biotech (Shanghai, China), and other chemical reagents for analysis on quality were purchased from Sinopharm Chemical Reagent (Shanghai, China). The cell culture medium and reagents were purchased from GIBCO (Shanghai, China). The HUVECs were purchased from ZhongQiaoXinZhou Biotechnology Co., Ltd. (Shanghai, China).

Cell culture medium and reagents were purchased from Gibco-BRL (Grand Island, NY, USA). Other experimental reagents (2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), hydrogen peroxide (H₂O₂), and formaldehyde) were obtained from Sigma-Aldrich Co. (Saint Louis, MO, USA), unless otherwise specified. All other chemicals used for analysis were of analytical grade and obtained from Guangzhou Analysis Test Center Keli Technology Development Co. (Guangdong, China).