Phorbol-12-myristate 13-acetate (PMA) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Dulbecco’s phosphate-buffered saline (DPBS), RPMI-1640 culture medium, and fetal bovine serum (FBS) were purchased from Welgene, Inc. (Daegu, Republic of Korea). Dopamine hydrochloride, RGD, and dexamethasone (Dex) were purchased from Sigma-Aldrich (St. Louis, MO, USA). SU-8 3005 and SU-8 developer were purchased from MicroChem (Round Rock, TX, USA). PDMS prepolymer (Sylgard 184) and a curing agent were purchased from Dow Corning (Midland, MI, USA). Norland Optical Adhesive (NOA) 63 was purchased from Norland Products (Jamesburg, NJ, USA). Tris-HCl was purchased from Biosesang (Seongnam, Republic of Korea). Ethylenediaminetetraacetic acid (EDTA) was purchased from Amresco Inc. (Solon, OH, USA). Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon (IFN)-γ were purchased from R&D Systems (Minneapolis, MN, USA). Pluronic F-127 solution and antibiotic–antimycotic solution were purchased from Invitrogen Corp. (Waltham, MA, USA). 1,25-dihydroxy vitamin D3 (VitD3) was purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada).
Pluronic f 127 solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
Pluronic F-127 solution is a non-ionic surfactant that forms thermoreversible gels. It is a triblock copolymer consisting of polyethylene oxide and polypropylene oxide segments. The solution exhibits temperature-dependent viscosity changes, making it suitable for various applications that require controlled rheological properties.
Automatically generated - may contain errors
Lab products found in correlation
Interferon ifn γ, by R&D Systems (1 mentions)
Dulbecco s phosphate buffered saline, by Welgene (1 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
Phorbol 12 myristate 13 acetate, by Cayman Chemical (1 mentions)
Rpmi 1640 culture medium, by Welgene (1 mentions)
Pdms prepolymer sylgard 184, by Dow (1 mentions)
Tris hcl, by Biosesang (1 mentions)
Dopamine hydrochloride, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Granulocyte macrophage colony stimulating factor gm csf, by R&D Systems (1 mentions)
Su 8 developer, by MicroChem (1 mentions)
Su 8 3005, by MicroChem (1 mentions)
Neutravidin, by Thermo Fisher Scientific (1 mentions)
2 protocols using pluronic f 127 solution
1
Generation of Immune Cell Cultures
2
Surface Passivation for Microtubule Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Flow chambers were washed with 200 µl H2O and 200 µl phosphate-buffered saline (PBS) and subsequently incubated with 30 µl of 5 mg/ml neutravidin (Invitrogen, dissolved in PBS) for 20 min as adapted from Mieck et al. (2015) . Unbound neutravidin was removed by washing with 50 µl PBS before the glass surface was passivated for 5 min with 1% Pluronic F-127 solution (Invitrogen, dissolved in PBS) (Mieck et al., 2015 ). Subsequently, each chamber was washed with 40 µl BRB80+ buffer (BRB80 supplemented with 2 mM DTT, 50 mM KCl, and 20 µM taxol).
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