Anti-PD-L1 (E1L3N) rabbit mAb (13,684), anti-CDK5 (2506S) and anti-ubiquitin (3933S) were purchased from Cell Signaling Technology. Mouse PD-L1 antibody (MAB90781-100) was purchased from R&D systems. Anti-TRIM21 (12,108-1-AP) was purchased from Proteintech. Fluorochrome-conjugated monoclonal antibodies including anti-CD3, anti-CD4, anti-CD8, anti-CD45, anti-PD-1 and live/dead were purchased from BD Pharmingen. In vivo mab anti-mouse PD-L1 was purchased from BioXcell. Cycloheximide (CHX) was purchased from Selleck. MG132 was purchased from MedChemExpress. Silver stain kit was purchased from Solarbio LIFE SCIENCES.
Anti trim21 12 108 1 ap
Manufactured by Proteintech
Sourced in China
Anti-TRIM21 (12,108-1-AP) is a rabbit polyclonal antibody that recognizes TRIM21, a protein involved in the ubiquitin-proteasome system. The antibody can be used for applications such as western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide (chx), by Selleck Chemicals (1 mentions)
Invivomab anti mouse pd l1, by BioXCell (1 mentions)
Mouse pd l1 antibody mab90781 100, by R&D Systems (1 mentions)
Anti ubiquitin 3933, by Cell Signaling Technology (1 mentions)
Anti cd45, by BD (1 mentions)
Anti cd8, by BD (1 mentions)
Anti cd4, by BD (1 mentions)
Anti pd 1, by BD (1 mentions)
Mg132, by MedChemExpress (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti mda5, by Cell Signaling Technology (1 mentions)
Anti grp94 9g10, by Enzo Life Sciences (1 mentions)
Anti viperin, by Cell Signaling Technology (1 mentions)
Anti rig 1, by Cell Signaling Technology (1 mentions)
Anti puromycin, by Merck Group (1 mentions)
Anti sting, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Anti rig 1 ap1900a 400, by Abcepta (1 mentions)
Anti trim25 h00007706 b01p, by Abnova (1 mentions)
Anti flag f1804, by Merck Group (1 mentions)
5 protocols using anti trim21 12 108 1 ap
1
Immunophenotyping and Protein Regulation Assay
2
Antibody Panel for Viral Immunology Research
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Primary antibodies used were mouse anti-HA (901502, Biolegend), rabbit anti-HA (51064-2-AP, Proteintech), anti-FLAG (F7425, Sigma), anti-puromycin (12D10, Sigma), anti-viperin (MaP.VIP) (44 (link)), anti-RIG-I (3743, Cell Signaling Technology), anti-MDA5 (5321, Cell Signaling Technology), anti-TRIM21 (12108-1-AP, Proteintech), anti-ISG15 (200401438, Rockland), anti-STING (13647, Cell Signaling Technology), anti-GRP94 (9G10, Enzo), and anti-GAPDH (10494-1-AP, Proteintech). Anti-SARS-CoV ORF3a was a kind gift from Carolyn Machamer (Johns Hopkins University, Baltimore, MD). All secondary antibodies used for immunofluorescence imaging and immunoblotting were purchased from Invitrogen and Jackson ImmunoResearch, respectively.
3
Antibody Panel for Western Blotting
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The following antibodies were used for Western blotting analysis in this study: anti-HA monoclonal antibody (MMS-101R, Covance, USA), anti-Myc monoclonal antibody (MMS-150P, Covance, Princeton, NJ, USA), anti-Tubulin (RM2003, Beijing Ray Antibody Biotech, Beijing, China), anti-Flag (F1804, Sigma, St. Louis, MO, USA), anti-RIG-I (AP1900A-400, Abgent, San Diego, CA, USA), anti-TRIM25 (H00007706-B01P, Abnova, Taiwan), anti-TRIM21 (12,108-1-AP, Proteintech, USA), anti-PCBP2 (15,070-1-AP, Proteintech, USA), anti-EV-D68 VP1 polyclonal antibody (GTX132313, GeneTex, USA), anti-CVA6 VP1 polyclonal antibody (GTX132346, GeneTex, USA). Polyclonal antibody (pAb) against EV71 and CVA16 viruses was obtained from rabbit immunized with EV71 or CVA16 whole viruses in our laboratory. The EV71 VP1 rabbit polyclonal antibody was produced in our lab.
4
Immunofluorescence Imaging of TRIM21 and ANXA2
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HUVECs were seeded onto slides overnight and fixed with 4% paraformaldehyde. Then, they were blocked for 1 h and incubated with anti-TRIM21 (12108–1-AP, Proteintech, China, 1:50) and anti-ANXA2 (sc-28385, Santa Cruz Biotechnology, Texas, USA, 1:50) antibodies. The next day, the cells were incubated with a fluorescent dye-labelled secondary antibody for 1 h. We washed the slides and added an anti-fluorescence attenuator containing DAPI. Images of stained cells were captured under a confocal microscopy. Pearson's R value of scatter plot analysis was calculated using ImageJ.
5
Antibody Reagents for Cell Signaling
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Anti‐YAP (D8H1X), anti‐MST1 (D8B9Q), anti‐MST2 (3952), anti‐p‐YAP (Ser 127) (D9W2I), anti‐LAST1 (9153), anti‐p‐LATS1 (9157), anti‐TAZ (E8E9G), anti‐p53 (DO‐7), and anti‐β‐catenin (D10A8) were purchased from Cell Signaling Technology (MA, USA). Anti‐GAPDH (10494‐1‐AP), anti‐TRIM21 (12108‐1‐AP), and anti‐MST1 (22245‐1‐AP) were purchased from Proteintech (Wuhan, China). Cycloheximide (CHX) and MG132 were purchased from Sigma Aldrich (MO, USA).
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