Cell supernatants were used for enzyme-linked immunoassay (ELISA). The supernatants were harvested, and 100µL of antibody dilutions (RELMβ, Abcam, Cambridge, UK; IL-1β, Abcam, Cambridge, UK; IL-8, Abcam, Cambridge, UK) were added to each well and incubated for 1 h. The secondary antibodies were added, and the reaction was terminated with the termination solution. A wavelength of 450 nm was used to measure the absorbance.
Relmβ
Manufactured by Abcam
Sourced in United Kingdom
RELMβ is a lab equipment product. It is a recombinant protein that belongs to the Resistin-like molecule family. The core function of RELMβ is to serve as a research tool for scientific investigations.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using relmβ
1
ELISA Quantification of Inflammatory Markers
2
ELISA and Western Blotting for Cytokine Analysis
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Cell supernatants were used for ELISA. The supernatants were harvested, and 100 µL of antibody dilutions (RELMβ, Abcam, Cambridge, UK; IL-1β, Abcam,Cambridge, UK; IL-8, Abcam, Cambridge, UK) were added to each well and incubated for 1 h. The secondary antibodies were added, and the reaction was terminated with the termination solution. A wavelength of 450 nm was used to measure the absorbance.
For the Western blotting assay, after the total protein concentration was determined, we separated equal amounts of proteins from each well by vertical electrophoresis using SDS-PAGE. We transferred the proteins to polyvinylidene fluoride membranes, followed by immunoblotting. IL-8 and IL-1β primary antibodies were purchased from Abcam (Cambridge, UK).
For the Western blotting assay, after the total protein concentration was determined, we separated equal amounts of proteins from each well by vertical electrophoresis using SDS-PAGE. We transferred the proteins to polyvinylidene fluoride membranes, followed by immunoblotting. IL-8 and IL-1β primary antibodies were purchased from Abcam (Cambridge, UK).
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