TCDD stock solution for zebrafish exposure was purchased at an initial concentration of 50 ± 0.32 μg/mL (Stock A) in dimethyl sulfoxide (DMSO, ED-901-B, Cambridge Isotope Laboratories, Tewksbury, MA). The stocks were made in 2 mL amber vials and caps were wrapped with Parafilm®. Dosing stocks were kept in the dark at room temperature. Dioxin mix, which contains TCDD, certified reference standard was purchased from AccuStandard for TCDD quantitation (New Haven, CT, USA). Surrogate standards (Polychlorinated Biphenyl (PCB) 65 and 166) and internal standard mix (Phenanthrene-D10, Chrysene-D12, and Carbon Number Distribution Marker) were also purchased from AccuStandard. Solvents, including n-hexane (≥99%), acetone (99.8%, HPLC grade), dichloromethane (99.8%, HPLC grade), water (UHPLC-MS grade), and acetonitrile (UHPLC-MS grade) were purchased from Fisher Scientific (Pittsburgh, PA). Isotopically labeled metabolites were purchased from Cambridge Isotopes (MSK-QC-KIT, Tewksbury, MA).
Isotopically labeled metabolites
Manufactured by Cambridge Isotopes
Isotopically labeled metabolites are chemically synthesized compounds that contain stable isotopes, such as 13C or 2H, at specific positions within the molecule. These isotopically labeled metabolites are used as analytical tools in various research applications, including metabolomics, tracer studies, and biochemical pathway analysis.
Automatically generated - may contain errors
Lab products found in correlation
Dichloromethane, by Thermo Fisher Scientific (2 mentions)
Acetone, by Thermo Fisher Scientific (2 mentions)
N hexane, by Thermo Fisher Scientific (2 mentions)
Ed 901 b, by Cambridge Isotopes (2 mentions)
Phenanthrene d10, by AccuStandard (2 mentions)
Chrysene d12, by AccuStandard (2 mentions)
Polychlorinated biphenyl pcb 65 and 166, by AccuStandard (2 mentions)
Carbon number distribution marker, by AccuStandard (2 mentions)
Water uhplc ms grade, by Thermo Fisher Scientific (2 mentions)
Acetonitrile uhplc ms grade, by Thermo Fisher Scientific (2 mentions)
2 protocols using isotopically labeled metabolites
1
Zebrafish TCDD Exposure Protocol
2
Zebrafish TCDD Exposure Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TCDD stock solution for zebrafish exposure was purchased at an initial concentration of 50 ± 0.32 μg/mL (Stock A) in dimethyl sulfoxide (DMSO, ED-901-B, Cambridge Isotope Laboratories, Tewksbury, MA). The stocks were made in 2 mL amber vials and caps were wrapped with Parafilm®. Dosing stocks were kept in the dark at room temperature. Dioxin mix, which contains TCDD, certified reference standard was purchased from AccuStandard for TCDD quantitation (New Haven, CT, USA). Surrogate standards (Polychlorinated Biphenyl (PCB) 65 and 166) and internal standard mix (Phenanthrene-D10, Chrysene-D12, and Carbon Number Distribution Marker) were also purchased from AccuStandard. Solvents, including n-hexane (≥99%), acetone (99.8%, HPLC grade), dichloromethane (99.8%, HPLC grade), water (UHPLC-MS grade), and acetonitrile (UHPLC-MS grade) were purchased from Fisher Scientific (Pittsburgh, PA). Isotopically labeled metabolites were purchased from Cambridge Isotopes (MSK-QC-KIT, Tewksbury, MA).
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