Male sprague dawley ofa rats

Male Sprague-Dawley OFA rats (Charles River Laboratories, L’Arbresle, France) weighing 200–220 g were used for the previously described NASH model^{5 (link)} and housed under 12 h light/dark cycle at constant temperature and humidity. Animals were fed ad libitum with a high fat glucose-fructose diet (HFGFD) or control diet (CD). HFGFD consisted of 30% fat (butter, coconut oil, palm oil, beef tallow) with mainly saturated fatty acids (5.73 Kcal/g), supplemented with cholesterol (1 g/Kg) (Ssniff Spezialdiaten GmbH, Soest, Germany), and a beverage of glucose-fructose (42 g/L, 45% glucose-55% fructose). Control diet consisted of a grain based chow that contents 4.8% fat (3.43 Kcal/g) (Safe-150, SAFE, Augy, France) and tap water. Body weight and food consumption was weekly monitored.
All procedures were conducted in accordance with European Union Guidelines for Ethical Care of Experimental Animals (EC Directive 86/609/EEC for animal experiments) and approved (file number: 9481) by the Animal Care Committee of the Vall d’Hebron Institut de Recerca (VHIR, Barcelona, Spain) and conducted in the animal facilities of VHIR.

All procedures were conducted in accordance with European Union Guidelines for Ethical Care of Experimental Animals (EC Directive 86/609/EEC for animal experiments) and approved (file number: 10,989) by the Animal Care Committee of the Vall d'Hebron Institut de Recerca (VHIR, Barcelona, Spain) and conducted in the animal facilities of VHIR.
Male Sprague-Dawley OFA rats (Charles River Laboratories, L'Arbresle, France) weighing 200-220 g were used for our previously described diet-induced NASH model (García-Lezana et al., 2018 (link)). Rats were housed under 12h light/dark cycle at constant temperature and humidity. They were fed ad libitum for 8 weeks with a HFGFD or CD. The HFGFD consisted of 30% fat (butter, coconut oil, palm oil and beef tallow), with mainly saturated fatty acids (5.73 Kcal/g), supplemented with cholesterol (1g/Kg) (Ssniff Spezialdiaten GmbH, Soest, Germany), and a beverage of glucose-fructose (42g/L, 45% glucose-55% fructose). The CD consisted of a grain-based chow that consisted of 4.8% fat (3.43 Kcal/g) (Safe-150, SAFE, Augy, France) and tap water. Body weight and food consumption were monitored weekly.