Tandem confocal microscope

Manufactured by Leica

The Tandem confocal microscope is a high-resolution imaging system designed for advanced microscopy applications. It utilizes a dual-channel confocal setup to capture detailed, high-contrast images of various samples. The core function of this microscope is to provide exceptional optical sectioning and three-dimensional visualization capabilities.

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Lab products found in correlation

Axiovert microscope, by Zeiss (2 mentions) Eclipse 50i microscope, by Nikon (1 mentions) Prolong gold mounting medium, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Vectashield solution containing dapi, by Vector Laboratories (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) γ tubulin, by Merck Group (1 mentions)

3 protocols using tandem confocal microscope

Multimodal Microscopy Imaging Protocol

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Phase contrast images of live cells were taken on a Zeiss Axiovert Microscope. Images of tumour histology were taken on a Nikon Eclipse 50i microscope. Immunofluorescent images of cells were taken on a Leica Tandem Confocal microscope using LAS-AF software.

Junaid F., Tomic G., Kemp R, & Winton D.J. (2023). Single-copy Snail upregulation causes partial epithelial-mesenchymal transition in colon cancer cells. BMC Cancer, 23, 153.

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Immunofluorescence Imaging of Cells

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Cells were grown on sterile glass cover slips. Before imaging the growth medium was removed and the cover slips washed in phosphate‐buffered saline (PBS) and then inverted onto a glass slide with a drop of Prolong® Gold mounting medium containing DAPI (4′,6‐diamidino‐2‐phenylindole) nuclear stain (Life Technologies), and left to dry in the dark. Slides were imaged using a Leica Tandem confocal microscope with an oil objective. Images were assembled as maximum projections from Z‐stacks of slices taken at 1‐μm intervals.

Patrick P.S., Rodrigues T.B., Kettunen M.I., Lyons S.K., Neves A.A, & Brindle K.M. (2015). Development of Timd2 as a reporter gene for MRI. Magnetic Resonance in Medicine, 75(4), 1697-1707.

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Immunofluorescence Staining for SIK2 and γ-Tubulin

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Cells were seeded at a density of 50,000 cells per well in a 24-well plate with coverslips. After at least 48 h to allow adherence, cells were washed in 1× PBS and were fixed with 4 % paraformaldehyde (Sigma-Aldrich) for 3 min. Cells were then washed in cold 1× PBS (Gibco), treated with 80 % ice cold ethanol and stored at −20°C until staining. For staining, cells were washed in 1× TBS-0.2 % Triton-0.04 % SDS, blocked for 30 min with 1× TBS supplemented with 1.5 % bovine serum albumin (BSA) (Sigma-Aldrich) and incubated for 1 h with a SIK2 (BioLegend) or γ-Tubulin (Sigma-Aldrich) primary antibody followed by an appropriate AlexaFluor488® or AlexaFluor594® conjugated secondary antibody (Invitrogen). Cover slips were then mounted onto slides with Vectashield solution containing DAPI (Vectashield). Images were taken using a Leica Tandem confocal microscope.

Bon H., Wadhwa K., Schreiner A., Osborne M., Carroll T., Ramos-Montoya A., Ross-Adams H., Visser M., Hoffmann R., Ahmed A.A., Neal D.E, & Mills I.G. (2014). Salt-inducible Kinase 2 Regulates Mitotic Progression and Transcription in Prostate Cancer. Molecular cancer research : MCR, 13(4), 620-635.

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