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Tandem confocal microscope

Manufactured by Leica

The Tandem confocal microscope is a high-resolution imaging system designed for advanced microscopy applications. It utilizes a dual-channel confocal setup to capture detailed, high-contrast images of various samples. The core function of this microscope is to provide exceptional optical sectioning and three-dimensional visualization capabilities.

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3 protocols using tandem confocal microscope

1

Multimodal Microscopy Imaging Protocol

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Phase contrast images of live cells were taken on a Zeiss Axiovert Microscope. Images of tumour histology were taken on a Nikon Eclipse 50i microscope. Immunofluorescent images of cells were taken on a Leica Tandem Confocal microscope using LAS-AF software.
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2

Immunofluorescence Imaging of Cells

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Cells were grown on sterile glass cover slips. Before imaging the growth medium was removed and the cover slips washed in phosphate‐buffered saline (PBS) and then inverted onto a glass slide with a drop of Prolong® Gold mounting medium containing DAPI (4′,6‐diamidino‐2‐phenylindole) nuclear stain (Life Technologies), and left to dry in the dark. Slides were imaged using a Leica Tandem confocal microscope with an oil objective. Images were assembled as maximum projections from Z‐stacks of slices taken at 1‐μm intervals.
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3

Immunofluorescence Staining for SIK2 and γ-Tubulin

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Cells were seeded at a density of 50,000 cells per well in a 24-well plate with coverslips. After at least 48 h to allow adherence, cells were washed in 1× PBS and were fixed with 4 % paraformaldehyde (Sigma-Aldrich) for 3 min. Cells were then washed in cold 1× PBS (Gibco), treated with 80 % ice cold ethanol and stored at −20°C until staining. For staining, cells were washed in 1× TBS-0.2 % Triton-0.04 % SDS, blocked for 30 min with 1× TBS supplemented with 1.5 % bovine serum albumin (BSA) (Sigma-Aldrich) and incubated for 1 h with a SIK2 (BioLegend) or γ-Tubulin (Sigma-Aldrich) primary antibody followed by an appropriate AlexaFluor488® or AlexaFluor594® conjugated secondary antibody (Invitrogen). Cover slips were then mounted onto slides with Vectashield solution containing DAPI (Vectashield). Images were taken using a Leica Tandem confocal microscope.
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