Passage 5 BMSCs were inoculated in 24‐well plates and grown in adipogenic differentiation basal medium A (Cyagen Biosciences). The medium was replaced with adipogenic differentiation basal medium B (Cyagen Biosciences) after 3 days, and cells grown for one more day. These steps were repeated up to day 14 (referred as the 3rd cycle). Cells were then fixed with 4% formalin solution and stained with Oil Red O (Yeasen) for 40 min followed by microscopic examination (Olympus).
Microscopic examination
Manufactured by Olympus
Sourced in China
The microscopic examination equipment from Olympus enables detailed analysis and observation of microscopic samples. It provides high-quality optics and advanced imaging capabilities to support scientific research and investigation.
Automatically generated - may contain errors
3 protocols using microscopic examination
1
Adipogenic Differentiation of BMSCs
2
Evaluating Cell Viability and Pathology
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VK2/E6E7, Vero, and HeLa cells were uniformly seeded in 96-well plates and subjected to HSV-2, JZ-1, or extracellular vesicles. Assessment of cell viability was conducted using the Cell Counting Kit-8 (CCK-8, Abbkine Scientific Co., Ltd, China), and microscopic examination (Olympus, Japan) was performed after a 24-h incubation to observe any pathological changes.
3
Aortic Lipid Deposition Evaluation
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Frozen sections of aorta were stained with Oil Red O (Solarbio, Beijing) to assess the lipid deposition. Sections were rinsed with 60% isopropanol, stained with Oil Red O for 10 min, differentiated with 60% isopropanol, and washed with water for 1-2 min. After counterstaining with hematoxylin for 3 min, microscopic examination (Olympus) was performed.
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