Cells were lysed in ice-cold whole-cell extract buffer B (50 mM Tris–HCl [pH 8.0], 4 M urea, and 1% Triton X-100) supplemented with complete protease inhibitor cocktail (Roche). The cell extracts were resolved with SDS-PAGE and analyzed with western blotting. The protein bands were visualized with ECL Blotting Detection Reagents. The antibodies used for western blotting included an anti-SRSF2 antibody (Santa Cruz Biotechnology, sc-10252), anti-H3K27Ac antibody (Abcam, ab4729), anti-Histone H3 antibody (Abcam, ab1791), and anti-β-actin antibody (Proteintech, 60008-1-Ig).
Sc 10252
Manufactured by Abcam
Sc-10252 is an antibody produced by Santa Cruz Biotechnology. It is a mouse monoclonal antibody targeting an unspecified antigen. The antibody is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Ab1791, by Proteintech (1 mentions)
Ab4729, by Abcam (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Ab11826, by Abcam (1 mentions)
Ab59240, by Abcam (1 mentions)
Ab70335, by Abcam (1 mentions)
Ab104238, by Abcam (1 mentions)
Dynabeads protein g immunoprecipitation kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using sc 10252
1
Whole-cell Protein Extraction and Western Blot
2
Immunoprecipitation of SRSF2 interactors
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The immunoprecipitation assay was performed using the Immunoprecipitation Protein G Dynabeads® kit (Invitrogen, 10007D) according to the manufacturer’s protocol. Briefly, HSV-1 or Mock infected HeLa cell lysates were incubated with antibodies against SRSF2 (Abcam, ab11826) and after washing, bound proteins were detected by western blotting with antibodies against PSPC1 (Abcam, ab104238), P54nrb (Abcam, ab70335), P300 (Abcam, ab59240), CBP (Abcam, ab50702), SRSF2 (Santa Cruz Biotechnology, sc-10252), EZH2 (Abcam, ab186006), or beta-actin (Proteintech, 60008-1-Ig).
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