Anti cav1

Manufactured by Merck Group

Sourced in United States

Anti-CAV1 is a laboratory equipment product designed for the detection and analysis of the Caveolin-1 (CAV1) protein. It is a research tool used in scientific investigations, but its specific intended use should not be extrapolated.

Automatically generated - may contain errors

Lab products found in correlation

Opti mem, by Thermo Fisher Scientific (2 mentions) Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions) Sodium citrate tribasic trihydrate s4641, by Merck Group (1 mentions) Fbs 16000 044, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti cbs, by Santa Cruz Biotechnology (1 mentions) Penn strep, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 10 040 cv, by Corning (1 mentions) Anti ki67, by Abcam (1 mentions) Anti actin, by Abcam (1 mentions) Anti cltc, by Merck Group (1 mentions) Anti rac1, by Thermo Fisher Scientific (1 mentions) Anti egfr, by Cell Signaling Technology (1 mentions) Odyssey system, by LI COR (1 mentions)

2 protocols using anti cav1

Lipid Nanoparticle Synthesis and Characterization

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DOTAP (890890P), DOPE (850725P), DOPC (850375P), and PE-PEG (880120P) were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). Tetrachloroauric acid (HAuCl₄·3H₂O) (520918) and sodium citrate tribasic trihydrate (S4641) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture media RPMI 1640 (10-040-CV) was obtained from Corning Inc. (Corning, NY, USA). FBS (16000-044) and Penn-Strep (15140-122) were purchased from Life Technologies (Grand Island, NY, USA), Opti-MEM was from Thermo Fisher Scientific (Waltham, MA, USA). The scrambled control siRNA (cat. SIC001), siRNAs against human MICU1 (SASI_Hs01_00070249), CBS (SASI_Hs01_00214623), and CAV1 (SASI_Hs01_00199504) were procured from Sigma-Aldrich. The following primary antibodies were purchased from the specified vendor: rabbit monoclonal anti-MICU1 (#12524, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-CBS (#sc-67154, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CAV1 (#SAB871521112, Sigma Aldrich), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich), and anti-Ki67 (no. ab833, Abcam).

Hossen M.N., Wang L., Chinthalapally H.R., Robertson J.D., Fung K.M., Wilhelm S., Bieniasz M., Bhattacharya R, & Mukherjee P. (2020). Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome. Science Advances, 6(30), eaba5379.

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Western Blot Protein Analysis

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Samples were run on a 10% polyacrylamide SDS-PAGE gel, transferred onto nitrocellulose membrane, and blocked for 30 min with 5% milk in TBS containing 0.1% Tween-20 (TBS-T). Membranes were probed with one of the following primary antibodies: 1:1000 anti-CLTC (Sigma, C1860), 1:1000 anti-CAV1 (Sigma, C4490) 1:1000 anti-Rac1 (ThermoFisher, PA1-091), 1:3000 anti-actin (abcam, ab6226), or 1:1000 anti-EGFR (Cell Signaling Tech, 4267). For standard experiments, membranes were washed with TBS-T, probed with goat anti-rabbit or goat anti-mouse HRP conjugated antibodies (Pierce), incubated with ECL substrate (Pierce) according to manufacturer’s instructions, and exposed to film. For quantitative experiments, membranes were probed with goat anti-mouse or donkey anti-goat IR Dye conjugated antibodies (Licor). Membranes were washed with TBS and signal was detected using a Licor Odyssey system.

Rinkenberger N, & Schoggins J.W. (2019). Comparative Analysis of Viral Entry for Asian and African Lineages of Zika Virus. Virology, 533, 59-67.

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