The 5 g of the dried and powder PCS was blended with 50 ml of the various solvents (80% methanol, 80% ethanol, 80% acetone and water) in a 250 ml volumetric flask. The mixtures were kept at room temperature for 8 h. In this regard, the extraction was performed during 15 min with magnetic stirring (8 s power on and 15 s power off in order to avoid super-boiling of solvent) with a 900 W microwave power. Then, the extract was filtered and a rotary evaporator (Buchi-V-800, Switzerland) was applied to remove the solvent at 50 °C under decreased pressure. In the end, a freeze-drier (Operon FDB-5503) was used to dry the produced extract at − 20 °C. The resulted extracts were stored in the freezer at − 18 °C for further testing [17 (link)].
V 800
Manufactured by Büchi
Sourced in Switzerland
The V-800 is a laboratory rotary evaporator designed for efficient solvent removal and concentration of liquid samples. It features a temperature-controlled heating bath and a rotational motion to facilitate the evaporation process. The core function of the V-800 is to provide a controlled and reliable solution for solvent evaporation in a laboratory setting.
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5 protocols using v 800
1
Optimized Microwave-Assisted Extraction
2
Enzymatic Polycondensation of Azelaic Acid and Glycerol
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The enzymatic polycondensations reactions were performed by using a thin‐film approach, as reported by Pellis et al.[6] Azelaic acid (5.0 g) and an equimolar amount of glycerol (2.4 g) were introduced into a 100 mL flask and different amount of lipases. The reactions were performed for 72 h at different temperatures and different values of the pressure and samples were withdrawn at 24 and 48 h. The reactions were performed using a Rotavapor R‐114 (BÜCHI) connected to a vacuum pump Vac® V‐500 (BÜCHI) and a pressure controllerV‐800 (BÜCHI). Temperature was controlled by means of a Waterbath B‐480 (BÜCHI). Samples were collected at pre‐set time intervals 24/48/72 h and analyzed by ESI‐MS spectrometry and NMR. At the end of reaction, methanol (40 mL) was added and the enzyme was removed by filtration and the product was recovered after methanol evaporation at 60 °C and stored at 4 °C until characterization without any further treatment. Control reactions, in the absence of enzyme were performed, and the formation of the product was not observed.
3
GC-FID Analysis of Volatile Compounds
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The volatile compounds from GPP were analysed using GC-FID. The 10 g of sample was extracted in 250 ml of dichloromethane for 2 h, afterward; the solvent was taken to evaporate using a rotary evaporator (V800, Büchi, Switzerland) at 40 °C under adjusted pressure at 900 mbar. The evaporated extract has adjusted the concentration of 10 mg/ml and filtered before the analysis with GC-FID (GC-2010, Shimadzu, Kyoto, Japan). One µl of the garlic-pepper solution was analysed using the GC-FID condition as described above15 ,16 (link).
4
Extraction of Ethanolic Plant Extracts
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The bark was washed and disinfected with a 2% NaClO solution. It was air-dried at room temperature for seven days. Then, it was submitted to a temperature of 40°C, in an oven with circulating air (Memmert 854, Schwabach, Germany), always verifying humidity loss (three days approximately). The cortex was ground to a fine powder and then weighed.
The crushed and weighed plant material was then subjected to cold maceration for three days in 96% ethanol. Successive concentrations of this extract were made at reduced pressure in a digital rotary evaporator (model R-124, vacuum controller V-800, Buchi) at 40°C to obtain an ethanolic extract, which was stored at 4°C until the phytochemical screening was performed.
The crushed and weighed plant material was then subjected to cold maceration for three days in 96% ethanol. Successive concentrations of this extract were made at reduced pressure in a digital rotary evaporator (model R-124, vacuum controller V-800, Buchi) at 40°C to obtain an ethanolic extract, which was stored at 4°C until the phytochemical screening was performed.
5
Extraction of Hibiscus sabdariffa Calyces
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Ten kilograms of dehydrated calyces of H. sabdariffa (“Criolla de Oaxaca” variety) grown in Oaxaca, Mexico were used in the study. The calyces were stored in a closed polyethylene container at room temperature until use. The acetonic extract from calyces of H. sabdariffa was obtained exactly as we previously described. Briefly, samples (100 g) of dehydrated calyces were placed in glass flasks and 900 mL of acetone were added (Sigma-Aldrich, Toluca, Mexico). The flasks were hermetically sealed and stored at room temperature for 7 days with manual shaking for 1 min once a day. After, the liquid phase was filtered through filter paper (Whatman Grade 4). The filtered extracts were concentrated in a rotary evaporator (V-800, Vacuum Controller, BÜCHI, Switzerland). The acetone was completely removed from the rotaevaporated concentrate by placing it in an air recirculation oven (Ambi-Hi-Low Chamber, Lab-Line, Jefferson, MO, USA) at 45 °C for 24 h [22 (link),31 (link)].
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