To further assess the ability of XN to reduce cellular proliferation, the colony-forming ability was determined by the measurement of the colonogenic cell survival. Cells were plated onto 6-well plates and treated with various concentrations of XN for 3 days. Then, media was removed and replaced with XN-free media. Following one week, media was again removed, and the cells were fixed with crystal violet staining and photographs were taken using Molecular Imager ChemiDoc XRS+ imager with image lab software (Bio-Rad, Hercules, CA). Colonies were then counted and compared to control.
Molecular imager chemidoc xrs imager
Manufactured by Bio-Rad
The Molecular Imager ChemiDoc XRS+ imager is a multipurpose imaging system designed for the detection and analysis of a wide range of molecular biology samples, including chemiluminescent, fluorescent, and colorimetric signals. The system utilizes a high-resolution camera and advanced optics to capture high-quality images of gels, blots, and other samples.
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2 protocols using molecular imager chemidoc xrs imager
1
Evaluating XN's Anti-Proliferative Effects
2
Colony Formation Assay for XN
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To further assess proliferation and efficacy of XN, colony formation assay was performed on these three cell lines. Cells were plated onto 6-well plates and allowed to adhere overnight. Cells were treated with DMSO (control), 5, 10, or 15 μM concentrations of XN for three days and then media was changed every 3 days without XN for 2 weeks. Cells were then fixed with crystal violet and images were obtained with Molecular Imager Chemi-Doc XRS+ imager with Image Lab software (Bio-Rad, Hercules, CA).
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