Hkbmm

IMR90, normal human fetal lung fibroblasts, and IOMM-Lee, a human malignant meningioma cell line expressing wild-type p53, were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% or 5% fetal bovine serum (FBS), respectively. HKBMM, a human malignant meningioma cell line expressing a mutant p53 (P177L), was obtained from the Riken BioResource Research Center (Tsukuba, Japan) and maintained in Ham’s F12 medium supplemented with 10% FBS. The culture media were supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin. The p53 status of IOMM-Lee and HKBMM cells was examined by cDNA sequencing as described below. IMR90 cells passaged fewer than 8 times were used in experiments. Detailed conditions for the in vitro drug treatment are described in the respective figure legends. In general, the day after cells had been passaged, the required amount of the drug stock solution was added to cells and gently mixed well. This was followed by an incubation at 37 °C in a 5% CO₂ incubator for the specified time, and cells were then subjected to each assay.