α rabbit hrp

Protein lysates were isolated from liver samples as described previously [9 (link)]. Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to PVDF membrane and analyzed by immunoblot with the following antibodies: α-NEMO, α-β-actin (Sigma Aldrich, St. Louis, MO, USA), α-cleaved Caspase-3, α-cleaved Caspase-8, α-IKKβ, p-JNK, JNK1 (Cell Signaling Technology, Danvers, MA, USA), α-IKKα, α-RIPK3 (Novus Biologicals, Centennials, CO, USA), α-PCNA (Thermo Fisher Scientific, Waltham, MA, USA), and α-GAPDH (Bio-Rad Laborories, Hercules, CA, USA). As secondary antibodies, α-rabbit-HRP, and α-mouse-HRP (GE Healthcare, Little Chalfont, UK) were used. Densitometry was performed by using ImageJ software (ImageJ, NIH, Bethesda, MD, USA).