Ysp 101

A stock solution of curcumin and PLGA in acetone was introduced into the device as dispersed phase through the inserted PTFE tubing (OD: 1/16" and ID: 1/32", OMNIFIT tubing). Syringe pumps (YSP-101 from YMC, Japan) mounted with gas-tight glass syringes were used to control the flow rate of the continuous (SGE Analytical Science, Australia) and dispersed phases (Hamilton, USA). The acetone was then removed under reduced pressure at 40 • C until the volume of the collected Cur-PLGA NP solution was reduced approximately by half to ensure complete removal of acetone. The resulting Cur-PLGA NP solution was kept refrigerated and filtered with a 200 nm syringe-driven filter (Millex from Merck, Germany) to ensure that there is no large aggregates in the sample before testing. The resulting Cur-PLGA solution was kept in the dark environment to limit the exposure to ambient light.
The in situ nanoprecipitation at the cross slot was imaged using spinning disk and structured illumination hybrid fluorescent confocal imaging (Andor DSD2, Japan) at 20x magnification on an inverted microscope (Ti-E, Nikon, Japan)

In this work, all experiments were conducted by using a capillary LC system constructed by a syringe pump YSP-101 (YMC, Kyoto, Japan) equipped with a gas-tight syringe (0.5 mL; Ito, Fuji, Japan) as a pump, an C4-1004-.2 internal sample injector (VICI Valco Instruments, Houston, USA) with an injection volume of 0.2 μL, a microcolumn prepared from a fused-silica capillary tube (100 × 0.32 mm i.d.; GL Sciences), a UV detector (JASCO, Tokyo, Japan) with the detection wavelength set at 210 nm, and a data processor (CDS-Lite Ver. 5.0; LA soft, Chiba, Japan). The inlet pressure was monitored by an L.TEX-8150 pressure sensor (L.TEX Corporation, Tokyo, Japan). Separation columns were operated under room temperature (controlled at 25°C) throughout the study.