Cardiac tissue or cardiomyocyte lysates were prepared using ice-cold RIPA buffer (Thermo Fisher Scientific) containing Complete Protease Inhibitor Cocktail and PhosSTOP (Roche). Lysates or culture medium were cleared by centrifugation, reduced, subjected to Western blot using a NuPage system (Invitrogen), and probed with antibodies against TGF-β 1/2/3 (Santa Cruz Biotechnology), TGF-β (Abcam), Troponin I (Abcam), Smad6 (ab13727, Abcam), phospho-Smad1/5 (Thermo Fisher), and phospho-Smad2/3 (sc-11769, Santa Cruz Biotechnology).
Tgf β 1 2 3
Manufactured by Santa Cruz Biotechnology
TGF-β 1/2/3 is a laboratory product offered by Santa Cruz Biotechnology. It is a set of recombinant transforming growth factor beta proteins that can be used in various research applications. The core function of these proteins is to facilitate the study of cellular signaling pathways involved in growth, differentiation, and other biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Ab13727, by Abcam (1 mentions)
Nupage system, by Thermo Fisher Scientific (1 mentions)
Phospho smad2 3, by Santa Cruz Biotechnology (1 mentions)
Sc 11769, by Santa Cruz Biotechnology (1 mentions)
Troponin 1, by Abcam (1 mentions)
Tgf β, by Abcam (1 mentions)
Phosphosmad1 5, by Thermo Fisher Scientific (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Phosstop, by Roche (1 mentions)
Control sirna a, by Santa Cruz Biotechnology (1 mentions)
C ebpβ, by Santa Cruz Biotechnology (1 mentions)
C ebp β, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
2 protocols using tgf β 1 2 3
1
Cardiac Tissue Protein Analysis
2
Silencing Key Regulators in M1 Macrophages
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Small interfering RNA for Sp1, β-arrestin 2, G-protein- coupled receptor kinase 2 (GRK2), transforming growth factor β1/2/3 (TGFβ1/2/3), C/EBPβ, transcriptional intermediary factor 1 β (TIF1β) or friend leukemia integration 1 (Fli-1) were obtained from Santa Cruz Biotechnology, Dallas, Texas. Transfection of M1 macrophages with siRNA for Sp1 (50 nM), β-arrestin 2 (50 nM), GRK2 (50 nM), TGFβ1/2/3 (50 nM), C/EBPβ (50 nM), TIF1β (50 nM) or Fli-1 (50 nM) was performed on days 7–8 of culture according to the manufacturer's protocol for Lipofectamine™ RNAiMAX (Life Technologies, Carlsbad, CA). Control siRNA-A (Santa Cruz Biotechnology) was utilized as the negative control for these experiments.
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