pWZL Blast mouse E-cadherin and pWZL Blast DN E-cadherin were from the Weinberg Lab (Addgene plasmids # 18804 and 18800, respectively). pRK5-Flag-HA-Merlin was from the Giancotti Lab (Addgene plasmid # 27104). 8xGTIIC-luciferase was from the Piccolo lab (Addgene plasmid # 34615). mCherry-TFR-20 was from the Davidson lab (Addgene plasmid # 55144). pQCXIH-Flag-YAP-S127A was from the Guan Lab (Addgene plasmid # 33092). pBABE-Flag-HA-Merlin was generated by PCR from pRK5-Flag-HA-Merlin (primers listed in Supplementary Table 2 ), digested by PacI and EcoRI FastDigest restriction enzymes (Thermo Fisher), and ligated into the empty pBABE-puro backbone using T4 ligase (NEB, Ipswich, MA, USA). FUW-tetO-Flag-HA-Merlin was created by digesting pRK5-Flag-HA-Merlin with EcoRI and XbaI and was ligated into the FUW-tetO-MCS vector from the Piccolo lab (Addgene plasmid # 84008). FUW-m2rtTA was from the Jaenisch lab (Addgene plasmid # 20342). PIK3CAH1047R was a gift from the Cantley lab (Weill Cornell Medicine, New York, NY).
Ecori fastdigest restriction enzymes
Manufactured by Thermo Fisher Scientific
Sourced in United States
EcoRI FastDigest restriction enzymes are DNA-cleaving enzymes that recognize and cleave the specific DNA sequence 5'-GAATTC-3'. These enzymes are designed to provide rapid and efficient DNA digestion, enabling researchers to perform their experiments more efficiently.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ecori fastdigest restriction enzymes
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Plasmid Construction and Sources
2
Plasmid Construction and Sources
Check if the same lab product or an alternative is used in the 5 most similar protocols
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pWZL Blast mouse E-cadherin and pWZL Blast DN E-cadherin were from the Weinberg Lab (Addgene plasmids # 18804 and 18800, respectively). pRK5-Flag-HA-Merlin was from the Giancotti Lab (Addgene plasmid # 27104). 8xGTIIC-luciferase was from the Piccolo lab (Addgene plasmid # 34615). mCherry-TFR-20 was from the Davidson lab (Addgene plasmid # 55144). pQCXIH-Flag-YAP-S127A was from the Guan Lab (Addgene plasmid # 33092). pBABE-Flag-HA-Merlin was generated by PCR from pRK5-Flag-HA-Merlin (primers listed in Supplementary Table 2 ), digested by PacI and EcoRI FastDigest restriction enzymes (Thermo Fisher), and ligated into the empty pBABE-puro backbone using T4 ligase (NEB, Ipswich, MA, USA). FUW-tetO-Flag-HA-Merlin was created by digesting pRK5-Flag-HA-Merlin with EcoRI and XbaI and was ligated into the FUW-tetO-MCS vector from the Piccolo lab (Addgene plasmid # 84008). FUW-m2rtTA was from the Jaenisch lab (Addgene plasmid # 20342). PIK3CAH1047R was a gift from the Cantley lab (Weill Cornell Medicine, New York, NY).
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