In Fig. 3E , Jurkat-PD-1 cells (2 × 105/200 μl/well) were seeded in 96-well plates in the presence of pretreated beads and cultured for 48 h. In Fig. 5D , Jurkat-PD-1 cells (2 × 105/200 μl/well) were seeded in 96-well plates in the presence of anti-CD3/CD28 activator (5 μl/ml) and exosomes and cultured for 48 h. Culture supernatants were then collected and the IL-2 concentration was measured using Human IL-2 ELISA MAX Deluxe (BioLegend).
Human il 2 elisa max deluxe
Manufactured by BioLegend
The Human IL-2 ELISA MAX Deluxe is a sandwich enzyme-linked immunosorbent assay (ELISA) kit designed to quantify human interleukin-2 (IL-2) levels in biological samples. It provides a sensitive and accurate method for the measurement of IL-2 concentrations.
Automatically generated - may contain errors
Lab products found in correlation
Human ifn gamma quantikine elisa kit, by R&D Systems (2 mentions)
Human tnf α elisa max deluxe, by BioLegend (2 mentions)
Human il 6 quantikine elisa kit, by R&D Systems (2 mentions)
Human il 10 duoset, by R&D Systems (1 mentions)
Cd4 and cd8 microbeads, by Miltenyi Biotec (1 mentions)
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Macs system, by Miltenyi Biotec (1 mentions)
Anti cd3 cd28 beads, by Thermo Fisher Scientific (1 mentions)
4 protocols using human il 2 elisa max deluxe
1
Jurkat-PD-1 Cells Activation Assay
2
Cytokine Profiling in NALM-6 Mouse Model
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The serum concentrations of human IL-2, IFN-γ, TNF-α, and IL-6 in NALM-6-bearing mice were measured using an enzyme-linked immunosorbent assay (ELISA). The following kits were used for each cytokine: Human IL-2 ELISA MAX Deluxe (BioLegend), Human IFN-gamma Quantikine ELISA Kit (R&D Systems), Human TNF-α ELISA MAX Deluxe (BioLegend), and Human IL-6 Quantikine ELISA Kit (R&D Systems). The concentration was calculated using a four-parameter logistic regression (4-PL) model.
3
Cytokine Profiling in NALM-6 Mouse Model
4
T Cell Cytokine Profiling After TIM-1 Stimulation
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TIM‐1‐expressing or mock vector was transfected into L cells, and supernatants were collected after culturing for 12 h. Peripheral blood mononuclear cells of three healthy donors were first separated from peripheral blood on a Ficoll‐paque density gradient (Sigma), and subsequently CD4+ and CD8+ T cells were collected using CD4 and CD8 MicroBeads and a magnetic‐activated cell sorting (MACS) system (Miltenyi Biotec, Bergisch‐Gladbach, Germany). CD4+ and CD8+ T cells were individually resuspended in the supernatants of L cells at a concentration of 1 × 106/mL, seeded in a 96‐well plate, and cultured under stimulation with anti‐CD3/CD28 beads (Life Technologies, Gaithersburg, MD). After 48 or 72 h of culture, cell numbers were counted and their supernatants were analyzed for cytokine production using the following ELISA kits: Human IL‐2 ELISA MAX Deluxe, Human IFN‐gamma ELISA MAX Standard, Human IL‐17A ELISA MAX Deluxe Set (Biolegend, CA), Human IL‐4 ELISA Set (Becton Dickinson, Mountainview, CA), and Human IL‐10 Duoset (R&D Systems).
+ Expand
TIM‐1‐expressing or mock vector was transfected into L cells, and supernatants were collected after culturing for 12 h. Peripheral blood mononuclear cells of three healthy donors were first separated from peripheral blood on a Ficoll‐paque density gradient (Sigma), and subsequently CD4+ and CD8+ T cells were collected using CD4 and CD8 MicroBeads and a magnetic‐activated cell sorting (MACS) system (Miltenyi Biotec, Bergisch‐Gladbach, Germany). CD4+ and CD8+ T cells were individually resuspended in the supernatants of L cells at a concentration of 1 × 106/mL, seeded in a 96‐well plate, and cultured under stimulation with anti‐CD3/CD28 beads (Life Technologies, Gaithersburg, MD). After 48 or 72 h of culture, cell numbers were counted and their supernatants were analyzed for cytokine production using the following ELISA kits: Human IL‐2 ELISA MAX Deluxe, Human IFN‐gamma ELISA MAX Standard, Human IL‐17A ELISA MAX Deluxe Set (Biolegend, CA), Human IL‐4 ELISA Set (Becton Dickinson, Mountainview, CA), and Human IL‐10 Duoset (R&D Systems).
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