Mouse embryonic fibroblast (MEF) cells were kindly provided by Dr. Randal J. Kaufman. Cells were maintained in DMEM (Corning, Corning city, NY, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Corning) and 1% (v/v) penicillin and streptomycin (P/S, Corning) at 37 °C in an incubator with 5% CO2. Cells were treated with thapsigargin (TG; Sigma Aldrich, St. Louis, MO, USA), 4μ8c (Cayman, Ann Arbor, MI, USA, 22110), or ceapinA7 (Sigma Aldrich) at the indicated concentrations and time points. MEFs expressing Fv2E-PERK were treated with AP20187 (B/B homodimerizer; Takara, Kusatsu, Shiga, Japan) at the indicated concentrations and time points.
Ap20187 b b homodimerizer
Manufactured by Takara Bio
Sourced in Japan
AP20187 is a B/B homodimerizer, a small molecule that can induce dimerization of proteins containing the B/B domain. The core function of AP20187 is to facilitate the study of protein-protein interactions and signaling pathways by enabling controlled dimerization of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Ceapin a7, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Thapsigargin tg, by Merck Group (1 mentions)
Anti gapdh antibody 2118, by Cell Signaling Technology (1 mentions)
Glutaraldehyde ga, by Electron Microscopy Sciences (1 mentions)
Bodipy 665 676, by Thermo Fisher Scientific (1 mentions)
Bodipy 493 503, by Thermo Fisher Scientific (1 mentions)
Dulbecco s pbs dpbs, by Thermo Fisher Scientific (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions)
2 protocols using ap20187 b b homodimerizer
1
Culturing and Treating Mouse Embryonic Fibroblasts
2
Fluorescent Protein Imaging and Antibody Detection
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BODIPY 493/503, BODIPY 665/676, BODIPY 581/591-C11, cumyl-OOH, anti-CHMP1B antibody (PA5-44773), and Dulbecco’s PBS (DPBS) were purchased from Thermo Fisher Scientific. NBD-C12, anti-ABCD1 antibody (ab197013), anti-PMP70 antibody (ab109448), anti-catalase antibody (ab110292), and anti-RFP antibody (ab124754) were obtained from Abcam. Oleic acid-BSA complex, nocodazole, anti-Spastin antibody (ABN368), anti-ACBD5 antibody (HPA012145), and DEUP were purchased from Sigma-Aldrich. Anti-GAPDH antibody (2118) was purchased from Cell Signaling. Anti-IST1 antibody (GTX101972) was obtained from GeneTex. PFA and glutaraldehyde (GA) were obtained from Electron Microscopy Science. AP20187 (B/B homodimerizer) was purchased from Takara. The recombinant anti-M1 Spastin rabbit monoclonal antibody, clone RM346 (31-1232-00: RevMAb Biosciences USA), was generated using RevMAb Biosciences’ proprietary B cell cloning technology against M1-specific sequences.
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