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Nefa detection kit

Manufactured by Nanjing Jiancheng
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Sourced in China

The NEFA Detection Kit is a laboratory equipment designed to detect and quantify non-esterified fatty acids (NEFAs) in various biological samples. It provides a reliable and efficient method for measuring NEFA levels, which is an important biomarker in the study of lipid metabolism and related health conditions.

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Lab products found in correlation

Tissue tg assay kit, by Applygen (1 mentions) Htx multi mode reader, by Agilent Technologies (1 mentions) Bca protein quantification kit, by Vazyme (1 mentions)

2 protocols using nefa detection kit

1

Quantifying lipid content in bMECs

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The miR-485 plasmids were transfected into bMECs, and then the TGs, CHOL, and NEFA contents were extracted 36 h later. We used a tissue TG Assay Kit (APPLYGEN, E1013, Beijing, China), Tissue CHOL Assay Kit (APPLYGEN, E1015, Beijing, China), NEFA Detection Kit (Nanjing Jiancheng Bioengineering Institute, A042-2-1, Nanjing, China), and BCA Protein Quantification Kit (Vazyme, E112-02, Nanjing, China) to quantify TGs, CHOL, and NEFA in bMECs. Finally, we used a microplate reader (SYNERGY HTX multi-mode reader, Bio Tek, S1LFTA) to measure the absorbance of the samples.
Liu J., Jiang P., Iqbal A., Ali S., Gao Z., Pan Z., Xia L., Yin F, & Zhao Z. (2021). MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells. Scientific Reports, 11, 7623.
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2

Quantifying Glucose Uptake and NEFA Release

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Glucose uptake was measured using the glucose oxidase-peroxidase technique with a glucose test kit (Rsbio, Shanghai, China). The discharge of NEFAs was tested using NEFA detection kit (Nanjing Jiancheng Institute of Bioengineering, Nanjing, China).
Liu T., Jin C., Sun J., Zhu L., Wang C., Xiao F., Liu X., Lv L., Yang X., Zhou W., Tan C., Wang X, & Wei W. (2024). Paroxetine alleviates dendritic cell and T lymphocyte activation via GRK2-mediated PI3K-AKT signaling in rheumatoid arthritis. Chinese medical journal.
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