2 2 diethoxyacetophenone deap

γ-Benzyl-L-glutamate (BLG), triphosgene, 4-dimethylaminopyridine (DMAP), trichloroacetic acid (TFA), N,N-dimethyl formamide (DMF) and tetrahydrofuran (THF) were purchased from Tianjin Heowns Biochem LLC. 1-Hydroxybenzotriazole (HOBT), 3-buten-1-amine, 2,2-diethoxyacetophenone (DEAP) and hydrobromic acid aqueous (48% HBr) were purchased from Sigma-Aldrich. Acryloyl chloride, acrylamide (AAm) and N,N-methylenebisacrylamide (BIS) were purchased from Innochem Co. Choline chloride (ChCl), ethylene glycol (EG), urea, glycerol, ethanedioic acid, glutaric acid and glucose were purchased from Aladdin.

Lipids used in this work are Egg PC l-α-phosphatidylcholine (T_t ≤ 0 °C, EPC), Hydro Soy PC l-α-phosphatidylcholine (T_t = 52 °C, HSPC), cholesterol (Chol), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (16:0 Liss RhodB-PE, λ_Ex/λ_Em = 560/583 nm) from Avanti Polar Lipids (Alabaster, AL, USA). N-isopropylacrylamide (NIPA) as a monomer, N,N′-methylene-bis-acrylamide (MBA) as a cross linker, fluorescein-o-acrylate (FA, λ_Ex/λ_Em = 490/520 nm) as a fluorescent monomer, ammonium persulfate (APS) as an initiator, N,N,N′,N′-tetramethylenediamine (TEMED) as an accelerator, sorbitane monostearate (Span 60) as a surfactant, 2,2-diethoxyacetophenone (DEAP) as a photoinitiator, and solvents (dimethyl sulfoxide, cyclohexane, chloroform, methanol, and ethanol of chemical grade) are purchased from Sigma Aldrich (Saint Louis, MO, USA). Water purified by RiOs-16 Essential Water Purification System (EMD Millipore, Billerica, MA, USA) at the resistivity of 16 MΩ·cm was used in all experiments. All chemicals are used as purchased without further purification.