Mouse monoclonal anti or antibody

The FinePure Universal DNA Purification Kit was supplied by GENFINE Biotech in Beijing, China. Taq plus master mix was purchased from Vazyme (Nanjing, China). Restriction enzymes, along with DO Supplement-Ura, all purchased from Takara Bio, Inc (Otsu, Shiga, Japan). Yeast strain YPH499 was obtained from the American Type Culture Collection (ATCC) in Virginia, USA. The growth mediums for yeast, specifically the yeast nitrogen base devoid of amino acids, along with dextrose, galactose, and MDZ, were supplied by Sigma-Aldrich (Missouri, USA). The rabbit polyclonal anti-CYP3A4 antibody was obtained from Abcam (Cambridge, UK) and the mouse monoclonal anti-OR antibody was from Santa Cruz Biotechnology in Dallas, Texas, USA.
The chemical 1-hydroxy Midazolam (1'-OH-MDZ) was obtained from Toronto Research Chemicals, Inc. (Toronto, Ontario, Canada). The NADPH-regenerating system was purchased from Promega in Madison, Wisconsin, USA. The Super Signal West Pico Trial Kit and highpressure liquid chromatography-grade solvents were acquired from Thermo Fisher Scientific Co.
in Waltham, Massachusetts, USA. All other chemicals and solvents used in our experiments were of the highest purity and analytical grade available on the market.