Sk ov 3 cells

PT and docetaxel (internal standard, IS) were gifted by Korea United Pharm, Inc. (Seoul, Korea). Isopropyl palmitate, palmitic, myristic and stearic acid were purchased from Daejung Chemical (Cheongwon, Korea). Tween 80, Tween 20, GMS, oleic acid, Span 85 and 80, phosphoric acid and mannitol were obtained Samchun Chemical (Pyungtaek, Korea). Capmul^® MCM-NF and -EP were purchased from Abitec Corporation (Columbus, OH, USA). Gelucire 44/14, 33/01, 43/01, 50/13, Capryol™ PGMC, Compritol^® 888 ATO, Capryol 90, Labrafac™ WL 1349 and CC, Labrasol, Cremophor^® EL, Labrafil^® M 2125 CS, Peceol, Lauroglycol 90, Transcutol^® HP, Lauroglycol™ FCC, precirol ATO, and Labrafil^® M 1944 CS were obtained by Gattefossé (Saint Priest, Cedex, France). Poloxamer 188, 407 and Solutol^® HS-15 were obtained from BASF (Ludwigshafen, Germany). Dimethyl sulfoxide (DMSO), DAPI, 3-(4,5-dimethylthoazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and rhodamine–phalloidin were obtained from Sigma-Aldrich (St. Louis, MO, USA). SK-OV-3 cells, an ovarian cancer cell line, were obtained from the Korean Cell Line Bank (Seoul, Korea). High-performance liquid chromatography (HPLC)-grade methanol (MeOH) and acetonitrile (ACN) were obtained from JT Baker (Phillipsburg, NJ, USA).

AGN was acquired from the market located in Pyeongchang (Korea). Poly(vinyl alcohol) (PVA, molecular weight: 30–70 kDa) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). RSV and Tween 80 were obtained from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Standard samples of D (purity: ≥95%) and DA (purity: ≥75%) were purchased from the Korea Promotion Institute for Traditional Medicine Industry (Gyeongsan, Korea). Phosphate buffered saline (PBS), RPMI 1640, fetal bovine serum (FBS), and penicillin-streptomycin were obtained from Gibco Life Technologies, Inc. (Carlsbad, CA, USA). SKOV-3 cells were acquired from the Korean Cell Line Bank (Seoul, Korea). The other chemicals were analytical grade and used without further purification.