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Xpb tfiih p89 s 19

Manufactured by Santa Cruz Biotechnology
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XPB (TFIIH p89 S-19) is a laboratory product offered by Santa Cruz Biotechnology. It is a component of the basal transcription factor TFIIH, which plays a crucial role in transcription initiation and nucleotide excision repair. The core function of this product is to provide a source of the XPB (TFIIH p89) protein for use in various research applications.

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Lab products found in correlation

Caspase glo reagent, by Promega (2 mentions) Celltiter glo, by Promega (2 mentions)

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XPB (S-19) (2 citations)

2 protocols using xpb tfiih p89 s 19

1

Viability, Caspase, and Protein Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in 96-well plates for viability assays and caspase activity assays, and in 96-, 24-, 12-, or 6-well plates for lysate collection for western blot experiments. Compounds in DMSO were added to achieve the indicated final concentration, for the indicated length of time. The same volume of DMSO was added as a negative control. Viability was measured using CellTiter Glo (Promega) following the manufacturer’s protocols, or caspase activity was measured using Caspase-Glo reagent (Promega) following manufacturer’s protocols, or cells were collected in hot 2x sample buffer, boiled, and sonicated for immunoblot analysis using standard western blot protocols. XPB (TFIIH p89 S-19, Santa Cruz) was used as a loading control.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
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2

Viability, Caspase, and Protein Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in 96-well plates for viability assays and caspase activity assays, and in 96-, 24-, 12-, or 6-well plates for lysate collection for western blot experiments. Compounds in DMSO were added to achieve the indicated final concentration, for the indicated length of time. The same volume of DMSO was added as a negative control. Viability was measured using CellTiter Glo (Promega) following the manufacturer’s protocols, or caspase activity was measured using Caspase-Glo reagent (Promega) following manufacturer’s protocols, or cells were collected in hot 2x sample buffer, boiled, and sonicated for immunoblot analysis using standard western blot protocols. XPB (TFIIH p89 S-19, Santa Cruz) was used as a loading control.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
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