Orbital Images were reconstructed in Arivis software (Zeiss, Rostock, Germany). EOMs were selected using the find large objects feature, and total volume of EOMs calculated. Graphs and statistical analysis (student’s t test) were done using GraphPad software.
Arivis software
Manufactured by Zeiss
Sourced in Germany
Arivis software is a comprehensive platform for processing and analyzing high-resolution microscopy data. It provides tools for image acquisition, visualization, and quantitative analysis of biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Axio zoom v16, by Zeiss (2 mentions)
Fluorescence stereomicroscope, by Zeiss (1 mentions)
Axiocam 506 color camera, by Zeiss (1 mentions)
Lightsheet z 1 microscope, by Zeiss (1 mentions)
Glass capillaries, by Zeiss (1 mentions)
Lightsheet microscope v1, by Zeiss (1 mentions)
Topvision low melting point agarose, by Thermo Fisher Scientific (1 mentions)
4 protocols using arivis software
1
Volumetric Analysis of Extraocular Muscles
2
Volumetric Analysis of Extraocular Muscles
3
In Vivo Zebrafish Tumor Imaging
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Live embryos were photographed at 1 h (t0, 48 hpf), 1 day (t1) and 4 days (t4) post implantation on agarose-coated dishes using an AxioZoom V16 fluorescence stereomicroscope (Zeiss, Oberkochen, Germany, EU) equipped with a digital Axiocam 506 color camera (Zeiss). The mean area of the tumor was manually measured using FIJI software [24 (link)]. Light sheet microscopy experiments were performed using a Light Sheet Z.1 microscope (Zeiss). For this purpose, t0, t1, and t4 embryos were embedded in a low melting agarose cylinder (1% low melting agarose:fish water, 1:1) and immersed in the observation chamber filled with fish water and anesthetic. Maximum intensity projections were obtained using the Zen software (Zeiss) and 3D reconstructions were made after z-stack processing with Arivis software (Zeiss).
To detect apoptotic cells, 48 hpf embryos were microinjected in the eye with 2.0 nL of a solution containing the anticancer drug under testing. After injection, zebrafish embryos were grown at 33 °C for 4 days. At t4, live embryos were soaked in fish water containing 2 μg/mL acridine orange and incubated at 28 °C for 20 min. After 8 washes for 5 min each with fish water, embryos were anesthetized and analyzed immediately with a fluorescence stereomicroscope (Zeiss).
To detect apoptotic cells, 48 hpf embryos were microinjected in the eye with 2.0 nL of a solution containing the anticancer drug under testing. After injection, zebrafish embryos were grown at 33 °C for 4 days. At t4, live embryos were soaked in fish water containing 2 μg/mL acridine orange and incubated at 28 °C for 20 min. After 8 washes for 5 min each with fish water, embryos were anesthetized and analyzed immediately with a fluorescence stereomicroscope (Zeiss).
4
Imaging Zebrafish Embryo Neurodevelopment
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Tg(neurod1:EGFP)ia50 zebrafish embryos at shield stage (6 hpf) were injected with vehicle (4 nanoliters of 10% DMSO in water) or with the maximal tolerated dose of GCP in vehicle (160 pmoles/embryo) as assessed by a series of preliminary experiments (data not shown). Then, embryos were first anesthetized using 0.16 mg/ml tricaine, embedded in 0.5% low melting agarose (Top Vision Low Melting Point Agarose, Thermo Fisher Scientific), and mounted on glass capillaries (Carl Zeiss, Germany). Images were acquired using Zeiss LightSheet microscope V1 supported by ZenPro software. EGFP acquisition was performed using 488–30 nm laser and 505–545 nm filter. Images from the same experiment were taken with the same laser intensity and exposure time to generate comparable images. After acquisition, 3D images were generated using Arivis software (Zeiss). 3D reconstructions were exported as a single snap with the same compression settings.
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