Human monocytes and platelets were isolated from healthy volunteers and lysed in RIPA buffer(27 (link)) (NaCl 150 mmol/L, Tris 50 mmol/L, SDS 1%, sodium deoxycholate 0.5%, Triton X-100 1%) containing proteinase inhibitor cocktail (Roche, Mannheim, Germany). For detection of CyPA, monocyte or platelet lysate or recombinant protein were run under reduced conditions in a 15% SDS-polyacrylamide gel, and CyPA was detected by using the TAKTE-mAbs as primary antibody or commercially available anti-CyPA antibody (abcam, Cambridge, UK). Bands were detected using a Li-Cor Odyssey infrared imaging system (LI-COR, Lincoln, NE, USA).
Anti cypa antibody
Manufactured by Abcam
Sourced in United States
The Anti-CyPA antibody is a primary antibody that specifically recognizes and binds to the Cyclophilin A (CyPA) protein, which is a ubiquitous and highly conserved enzyme involved in various cellular processes. This antibody can be used for the detection and analysis of CyPA in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescence reagent, by Cytiva (2 mentions)
Anti p38 mapk antibody, by Cell Signaling Technology (2 mentions)
Anti cd147 antibody, by Abcam (2 mentions)
Anti e cadherin antibody, by Abcam (2 mentions)
Anti fn antibody, by Abcam (2 mentions)
Anti phospho p38 mapk antibody, by Cell Signaling Technology (2 mentions)
Anti α sma antibody, by Abcam (2 mentions)
Goat anti rabbit igg, by Abcam (2 mentions)
Anti gapdh antibody, by Abcam (2 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Proteinase inhibitor cocktail, by Roche (1 mentions)
Fluorescence microscopy, by Leica camera (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Nitrocellulose membrane, by Cytiva (1 mentions)
4 protocols using anti cypa antibody
1
Detecting Cyclophilin A in Monocytes and Platelets
2
Immunofluorescence Localization of CyPA in Uterus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded uteri sections (5 μm thick) were deparaffinized and dehydrated. Sections were blocked in 10% horse serum for 1 h at 37°C and then incubated with anti-CyPA antibody (final concentration: 10 µg/mL; Abcam) overnight at 4°C. After washing, the sections were incubated with anti-goat Alexa Fluor 488 (final concentration: 10 µg/mL, Invitrogen) as the second antibody for 1 h and counterstained with DAPI for nuclei. Finally, the fluorescence signals were observed under fluorescence microscopy (Leica).
3
Quantitative Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Western Blot assay was performed as previously reported [24 (link)]. Briefly, total proteins were extracted from the rat kidney tissues and HK-2 cells from each group. Samples were separated by 10% SDS-PAGE and then transferred into a nitrocellulose membrane (Whatman, Florham Park, NJ). The membrane was blocked using 5% dry fat-free milk in PBS with 0.1% Tween for 60 min. at room temperature, and then incubated with primary antibodies overnight which are shown as follows: anti-α-SMA antibody (1:1000; Abcam, USA); anti-E-cadherin antibody (1:1000; Abcam, USA); anti-FN antibody (1:1000; Abcam, USA); anti-CD147 antibody (1:1000; Abcam, USA); anti-CyPA antibody (1:1000; Abcam, USA); anti-GAPDH antibody (1:1000; Abcam, USA); anti-phospho-p38 MAPK antibody (1:1000; Cell Signaling Technology, USA); and anti-p38 MAPK antibody (1:1000; Cell Signaling Technology, USA). The secondary antibody used was a goat anti-rabbit IgG (1:4000; Abcam, USA). The proteins were visualized by chemiluminescence reagents (Amersham Biosciences, USA). All the experiments were carried out at least three times.
4
Western Blot Analysis of Kidney Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The detailed procedures of Western Blot assay were described as previously reported (12) . Brie y, total proteins were extracted from the rat kidney tissues and HK-2 cells derived from each group. Primary antibodies used in this study were shown as below: anti-α-SMA antibody (1:1000; Abcam, USA); anti-E-Cadherin antibody (1:1000; Abcam, USA); anti-FN antibody (1:1000; Abcam, USA); anti-CD147 antibody (1:1000; Abcam, USA); anti-CyPA antibody (1:1000; Abcam, USA); anti-GAPDH antibody (1:1000; Abcam, USA); anti-phospho-p38 MAPK antibody (1:1000; Cell Signaling Technology, USA); anti-p38 MAPK antibody (1:1000; Cell Signaling Technology, USA). The secondary antibody in this study was goat antirabbit IgG (1:4000; Abcam, USA). The proteins were visualized by chemiluminescence reagents (Amersham Biosciences, USA). All the experiments above were carried out for at least three times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!