EKP isolates were identified by the Gram-Negative-Identification Card of the Vitek system (bioMe’rieux, Lyon, France). During the study period, these isolated from blood culture were prospectively stored. Cefazolin MICs was manually determined by the broth microdilution method. Susceptibility to empirical antimicrobials was tested by the disk diffusion method. The antimicrobial susceptibility was interpreted based on contemporary CLSI breakpoints [12 ].
Gram negative identification card
Manufactured by bioMérieux
Sourced in France
The Gram-Negative-Identification Card is a laboratory equipment product designed to identify and differentiate gram-negative bacteria. It provides a rapid and reliable method for the identification of various gram-negative species.
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Lab products found in correlation
2 protocols using gram negative identification card
1
Antibiotic Susceptibility of Gram-Negative Isolates
2
Isolation and Identification of E. coli
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A total of 246 clinical isolates were included from patients with E. coli infections from Prince Hamzah Hospital and Islamic Hospital in Amman, Jordan, from October 2017 to December 2019. All isolates were collected after obtaining voluntary consent and ethical approval. Isolates were identified by standard microbiological procedures including culture on MacConkey agar, Gram stain, and manual biochemical tests, including citrate, indole, methyl-red, and voges-proskauer tests. Furthermore, species confirmation was carried out using the Vitek 2 compact system, using a Gram-negative identification card (BioMerieux, France).
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