Anti cd19

Manufactured by Biocare Medical

Anti-CD19 is a monoclonal antibody that binds to the CD19 antigen expressed on B cells. It is a laboratory research tool used for the identification and analysis of B cells in biological samples.

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Lab products found in correlation

Anti bcl6, by Biocare Medical (2 mentions) Anti igd, by Agilent Technologies (2 mentions) Anti cd27, by Abcam (2 mentions) Opal multiplex kit, by PerkinElmer (2 mentions) Anti aid, by Thermo Fisher Scientific (1 mentions) Anti cd3, by Agilent Technologies (1 mentions) Gata3, by Biocare Medical (1 mentions) Anti cd8, by Abcam (1 mentions) Anti cd4, by Abcam (1 mentions) Prolong diamond antifade mountant containing dapi, by Thermo Fisher Scientific (1 mentions) Anti tnf α, by Abcam (1 mentions) Anti cd11c, by Abcam (1 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) Anti cd4, by Biocare Medical (1 mentions)

2 protocols using anti cd19

Multiplex Immunohistochemistry for Immune Cell Profiling

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Tissue samples were fixed in formalin, embedded in paraffin, and sectioned. These specimens were incubated with the following antibodies: anti-CD3 (clone: A045229-2; DAKO), anti-CD4 (clone: EPR6855;Abcam), anti-CD19 (clone: SKU310; Biocare Medical), anti-Bcl6 (clone: LN22; Biocare Medical), anti-AID (clone: ZA001; Invitrogen), anti-T-bet (clone: ab150440; Abcam), GATA3 (clone: CM405A; Biocare), ICOS (clone: 89601; Cell Signaling Technology), Rorc (clone: ab212496; Abcam), CXCR5 (clone: MAB190; R&D Systems), Foxp3 (clone: 98377; Cell Signaling Technology), anti-CD8 (clone: ab85792; Abcam), anti-IgD (clone: AA093; DAKO), anti-CD27 (clone: ab131254; Abcam), anti-IgG (clone: ab109489; Abcam), anti-TNF-α (clone: ab6671; Abcam), and anti-CD35 (clone: ab25; Abcam) followed by incubation with a secondary antibody using an Opal Multiplex Kit (Perkin Elmer). The samples were mounted with ProLong Diamond Antifade mountant containing DAPI (Invitrogen).

Kaneko N., Kuo H.H., Boucau J., Farmer J.R., Allard-Chamard H., Mahajan V.S., Piechocka-Trocha A., Lefteri K., Osborn M., Bals J., Bartsch Y.C., Bonheur N., Caradonna T.M., Chevalier J., Chowdhury F., Diefenbach T.J., Einkauf K., Fallon J., Feldman J., Finn K.K., Garcia-Broncano P., Hartana C.A., Hauser B.M., Jiang C., Kaplonek P., Karpell M., Koscher E.C., Lian X., Liu H., Liu J., Ly N.L., Michell A.R., Rassadkina Y., Seiger K., Sessa L., Shin S., Singh N., Sun W., Sun X., Ticheli H.J., Waring M.T., Zhu A.L., Alter G., Li J.Z., Lingwood D., Schmidt A.G., Lichterfeld M., Walker B.D., Yu X.G., Padera RF J.r, & Pillai S. (2020). Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19. Cell, 183(1), 143-157.e13.

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Multiplex Immunohistochemistry of Immune Markers

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Tissue samples were fixed in formalin, embedded in paraffin, and sectioned. These specimens were incubated with the following antibodies: anti-CD4 (clone: CM153A; Biocare Medical), anti-CD19 (clone: SKU310; Biocare Medical), anti-CD20 (clone: LN26; Novus Biological), anti-Bcl6 (clone: LN22; Biocare Medical), anti-IgD (clone: AA093; DAKO), anti-CD27 (clone: ab131254; Abcam), anti-IgG (clone: ab109489; Abcam), anti-SLAMF7 (clone: HPA055945; Sigma-Aldrich) and anti-CD11c (clone: ab52632; Abcam) followed by incubation with a secondary antibody using an Opal™ Multiplex Kit (Perkin Elmer). The samples were mounted with ProLong™ Diamond Antifade mountant containing DAPI (Invitrogen).

Allard-Chamard H., Kaneko N., Bertocchi A., Sun N., Boucau J., Kuo H.H., Farmer J.R., Perugino C., Mahajan V.S., Murphy S.J., Premo K., Diefenbach T., Ghebremichael M., Yuen G., Kotta A., Akman Z., Lichterfeld M., Walker B.D., Yu X.G., Moriyama M., Maehara T., Nakamura S., Stone J.H., Padera R.F, & Pillai S. (2023). Extrafollicular IgD−CD27−CXCR5−CD11c− DN3 B cells infiltrate inflamed tissues in autoimmune fibrosis and in severe COVID-19. Cell Reports, 42(6), 112630.

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