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Superscript 3 first strand rt pcr

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Superscript III First-Strand RT-PCR is a reverse transcriptase enzyme for first-strand cDNA synthesis. It is designed for high-efficiency reverse transcription of RNA templates.

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Superscript III (4906 citations) SuperScript III First-Strand Synthesis System for RT-PCR (589 citations) SuperScript III RT (445 citations) SuperScript III First-Strand (94 citations) SuperScript III First-Strand Synthesis System for RT-PCR kit (71 citations) Superscript III First Strand RT-PCR kit (25 citations) Superscript III First Strand Synthesis kit for RT-PCR (17 citations) SuperScript III First-Strand System for RT-PCR (7 citations) Superscript III First-Strand Synthesis SuperMix for RT-PCR (7 citations) SuperScript III First-Strand Synthesis for RT-PCR (6 citations)

2 protocols using superscript 3 first strand rt pcr

1

Quantitative RT-PCR of Patient RNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA synthesis of 1 μg patient RNA was performed with Superscript III First-Strand RT-PCR (Life Technologies). The reaction products were then used for quantitative real-time PCR (qRT-PCR) or cloning. qRT-PCR was performed in triplicate on 10 ng human cDNA for CTNNA2, AXIN2, ID2, LEF1, and GAPDH (see Supplementary Table 1 for primer sequences). CT values were normalized to GAPDH as a loading control and fold change calculated in reference.
Schaffer A.E., Breuss M.W., Caglayan A.O., Al-Sanaa N., Al-Abdulwahed H.Y., Kaymakçalan H., Yılmaz C., Zaki M.S., Rosti R.O., Copeland B., Baek S.T., Musaev D., Scott E.C., Ben-Omran T., Kariminejad A., Kayserili H., Mojahedi F., Kara M., Cai N., Silhavy J.L., Elsharif S., Fenercioglu E., Barshop B.A., Kara B., Wang R., Stanley V., James K.N., Nachnani R., Kalur A., Megahed H., Incecik F., Danda S., Alanay Y., Faqeih E., Melikishvili G., Mansour L., Miller I., Sukhudyan B., Chelly J., Dobyns W.B., Bilguvar K., Jamra R.A., Gunel M, & Gleeson J.G. (2018). Bi-allelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 over-activity and disordered cortical neuronal migration. Nature genetics, 50(8), 1093-1101.
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2

Quantitative RT-PCR of Patient RNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA synthesis of 1 μg patient RNA was performed with Superscript III First-Strand RT-PCR (Life Technologies). The reaction products were then used for quantitative real-time PCR (qRT-PCR) or cloning. qRT-PCR was performed in triplicate on 10 ng human cDNA for CTNNA2, AXIN2, ID2, LEF1, and GAPDH (see Supplementary Table 1 for primer sequences). CT values were normalized to GAPDH as a loading control and fold change calculated in reference.
Schaffer A.E., Breuss M.W., Caglayan A.O., Al-Sanaa N., Al-Abdulwahed H.Y., Kaymakçalan H., Yılmaz C., Zaki M.S., Rosti R.O., Copeland B., Baek S.T., Musaev D., Scott E.C., Ben-Omran T., Kariminejad A., Kayserili H., Mojahedi F., Kara M., Cai N., Silhavy J.L., Elsharif S., Fenercioglu E., Barshop B.A., Kara B., Wang R., Stanley V., James K.N., Nachnani R., Kalur A., Megahed H., Incecik F., Danda S., Alanay Y., Faqeih E., Melikishvili G., Mansour L., Miller I., Sukhudyan B., Chelly J., Dobyns W.B., Bilguvar K., Jamra R.A., Gunel M, & Gleeson J.G. (2018). Bi-allelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 over-activity and disordered cortical neuronal migration. Nature genetics, 50(8), 1093-1101.
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