Recombinant human tPA (Actilyse®) was purchased from Boehringer Ingelheim (Ingelheim am Rhein, Germany). Fetal bovine serum, horse serum, lipofectamine R 2000 reagent, B27 supplement, glutamine, laminin, neurobasal medium, and penicillin/streptomycin were purchased from ThermoFisher (Waltham, Massachusetts, USA). Dulbecco’s modified Eagle’s medium (DMEM), poly-D-lysine, phosphate-buffered saline (PBS), Glycine (Gly), paraformaldehyde, albumin from bovine serum, ammonium chloride (NH4Cl), potassium chloride (KCl), and rabbit polyclonal antibody were purchased from Sigma-Aldrich (St Louis, MO, USA). Tetrodotoxin citrate (TTX), cyanquixaline (CNQX), (2R)-amino-5-phosphonovaleric acid (APV) and bicuculline methiodide (Bic) were purchased from Tocris (Bristol, UK). HaloTag® TMR ligand was purchased from Promega (Madison, Wisconsin, USA). The following primary antibodies were used for immunocytochemistry: mouse monoclonal anti-HaloTag® (dilution 1:1,000; Promega; G9211) rabbit anti-tPA polyclonal antibody (dilution 1:1 500; generous gift from R. Lijnen, Leuven), chicken anti-Microtubule-associated protein 2 (MAP2) polyclonal antibody (dilution 1:8 000; Abcam, Cambridge, UK; ab5392). Secondary fluorescent antibodies (Alexa647; dilution 1:800) were purchased from Jackson Immunoresearch (Bar Harbor, ME, USA).
Bicuculline methiodide bic
Manufactured by Bio-Techne
Sourced in United Kingdom
Bicuculline methiodide (Bic) is a chemical compound commonly used in scientific research. It is a GABA antagonist, a class of molecules that block the inhibitory effects of the neurotransmitter GABA in the central nervous system.
Automatically generated - may contain errors
Lab products found in correlation
6 cyano 7 nitroquinoxaline 2 3 dione cnqx, by Bio-Techne (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Neurobasal medium, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
B27 supplement, by Thermo Fisher Scientific (2 mentions)
Laminin, by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Halotag tmr ligand, by Promega (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Lipofectamine r 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Anti halotag, by Promega (1 mentions)
Albumin from bovine serum, by Merck Group (1 mentions)
Way100635, by Bio-Techne (1 mentions)
Sb242084, by Bio-Techne (1 mentions)
8 oh dpat, by Bio-Techne (1 mentions)
As 19, by Bio-Techne (1 mentions)
Sb 269970, by Bio-Techne (1 mentions)
Sb258585, by Bio-Techne (1 mentions)
Serotonin hydrochloride 5 ht, by Merck Group (1 mentions)
Orexin a oxa, by Bachem (1 mentions)
3 protocols using bicuculline methiodide bic
1
Immunocytochemical Labeling of tPA Expressing Neurons
2
Pharmacological modulation of neural activity
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AS19 (#1968), SB242084 (2901/10), SB269970 (#1612/10), SB258585 (#1961/10), WAY100635 (#4380), 8-OH-DPAT (#0529/10), WAY629 (#2173), 5-Carboxamidotryptamine meleate (5-CT, #0458),
D-2-amino-5-phosphonovalerate (AP5, #0106), bicuculline methiodide (Bic, #2503), and 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, #0190) were purchased from Tocris Bioscience. Serotonin hydrochloride (5-HT, #H9523) was obtained from Sigma–Aldrich. All drugs were dissolved in water or DMSO and aliquoted as stock solutions that were stored at −80 °C. On the day of recording, the stock solutions were diluted to final concentrations in ACSF (at least 1:1000 dilution) for the experimental test. For experiments when DMSO was used for preparing stock solutions of some drugs (AS19, SB242084, and CNQX), we also added 0.1% of DMSO in ACSF of control condition. During the recording, drugs were delivered locally via a multi-channel drug application system (Warner Instruments, CT, USA) with a flow pipette of 250-μM diameter so that the drug solutions were quickly washed out after the drug channel was switched to normal ACSF.
D-2-amino-5-phosphonovalerate (AP5, #0106), bicuculline methiodide (Bic, #2503), and 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, #0190) were purchased from Tocris Bioscience. Serotonin hydrochloride (5-HT, #H9523) was obtained from Sigma–Aldrich. All drugs were dissolved in water or DMSO and aliquoted as stock solutions that were stored at −80 °C. On the day of recording, the stock solutions were diluted to final concentrations in ACSF (at least 1:1000 dilution) for the experimental test. For experiments when DMSO was used for preparing stock solutions of some drugs (AS19, SB242084, and CNQX), we also added 0.1% of DMSO in ACSF of control condition. During the recording, drugs were delivered locally via a multi-channel drug application system (Warner Instruments, CT, USA) with a flow pipette of 250-μM diameter so that the drug solutions were quickly washed out after the drug channel was switched to normal ACSF.
3
Electrophysiological Recording of Orexin and GLP-1 Signaling
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Orexin A (OXA; 200 nM, Bachem, Switzerland), glucagon-like peptide 1 (GLP-1; 1µM, Bachem), 6cyano-7-nitroquinoxaline-2,3-dione (CNQX; 10 µM, Tocris, UK), DL-2-Amino-5-phosphonopentanoic acid (DL-AP5, 40 µM, Tocris), bicuculline methiodide (Bic, 20 μM, Tocris), tetrodotoxin citrate (TTX; 0.5 µM, Tocris) were stocked at 100x concentration at -20°C and were freshly diluted in the recording ACSF prior the application by bath perfusion.
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