Cgs 3 multiangle goniometer

Manufactured by Malvern Panalytical

Sourced in United Kingdom

The CGS-3 multiangle goniometer is a laboratory instrument designed for the measurement of particle size and shape. The core function of the CGS-3 is to accurately determine the size distribution and morphology of particles in a sample using light scattering principles.

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Lab products found in correlation

Alv 60x0 software v 3 x, by Malvern Panalytical (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions)

3 protocols using cgs 3 multiangle goniometer

Dynamic Light Scattering Analysis of Particle Size

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The size and the size distribution
of empty particles were measured by dynamic light scattering (DLS)
using a Malvern CGS-3 multiangle goniometer (Malvern ltd., Malvern),
consisting of a HeNe laser source (λ = 632.8 nm, 22 mW output
power), temperature controller (Julabo water bath) and a digital correlator
ALV-5000/EPP. Time correlation functions were analyzed using the ALV-60X0
Software v 3.X provided by Malvern, to obtain the Z-average hydrodynamic diameter of the particles (Zave) and the particle
size distribution (polydispersity index, PDI). The samples were analyzed
at 25 °C.

van Lith S.A., van Duijnhoven S.M., Navis A.C., Leenders W.P., Dolk E., Wennink J.W., van Nostrum C.F, & van Hest J.C. (2017). Legomedicine—A Versatile Chemo-Enzymatic Approach for the Preparation of Targeted Dual-Labeled Llama Antibody–Nanoparticle Conjugates. Bioconjugate Chemistry, 28(2), 539-548.

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Liposome Particle Size Characterization

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The mean particle size and polydispersity index (PDI) were measured by dynamic light scattering (DLS) with a Malvern CGS-3 multiangle goniometer with He–Ne laser source (λ = 632.8 nm, 22 mW output power) using an angle of 90° (Malvern Instruments, Malvern, UK). For the measurement, liposome samples were diluted to 0.25 mM (lipid concentration) in 20 mM HEPES with 140 mM NaCl, pH 7.4.

Kontturi L.S., van den Dikkenberg J., Urtti A., Hennink W.E, & Mastrobattista E. (2019). Light-Triggered Cellular Delivery of Oligonucleotides. Pharmaceutics, 11(2), 90.

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Liposomal Lipid-Nucleic Acid Separation

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To separate the lipids from the NA, samples are extracted according to Bligh and Dyer. 32 The top phase of the extraction was collected and evaporated using a centrifugal concentrator.
When completely dry, the samples were reconstituted in MilliQ water and the amount of nucleic acid was measured by UV/VIS spectrophotometry at 260 nm using a NanoDrop 1000 spectrophotometer (Thermo Scientific). The lipid fraction in chloroform was assayed for the total phosphate amount according to the method of Rouser using sodium biphosphate as a standard. 33 The total lipid amount was then extrapolated from the amount of phospholipid (DOPE) present in the formulation. The encapsulation efficiency was calculated using the formula
The hydrodynamic diameter and the polydispersity index were measured by dynamic light scattering, using a Malvern CGS-3 multiangle goniometer with a He-Ne laser source (λ = 632.8 nm, 22 mW output power) under an angle of 90°( Malvern Instruments, Malvern, UK). The zeta-potential of the liposomes was measured using laser Doppler electrophoresis on a Zetasizer Nano-Z (Malvern Instruments) with samples dispersed in 10 mM Hepes buffer pH 7.4 (no additional salts).

Oude Blenke E.E., van den Dikkenberg J., van Kolck B., Kros A, & Mastrobattista E. (2016). Coiled coil interactions for the targeting of liposomes for nucleic acid delivery. Nanoscale, 8(16).

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